Elucidation of the mechanisms underlying the angiogenic effects of ginsenoside Rg1 in vivo and in vitro

Patrick Y K YUE, Daisy Y.L. Wong, W. Y. Ha, M. C. Fung, Nai Ki MAK, H. W. Yeung, H. W. Leung, Kelvin Chan, L. Liu, T. P.D. Fan, Ricky N S WONG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

The major active constituents of ginseng are ginsenosides, and Rg 1 is a predominant compound of the total extract. Recent studies have demonstrated that Rg1 can promote angiogenesis in vivo and in vitro. In this study, we used a DNA microarray technology to elucidate the mechanisms of action of Rg1. We report that Rg1 induces the proliferation of HUVECs, monitored using [3H]-thymidine incorporation and Trypan blue exclusion assays. Furthermore, Rg1 (150-600 nM) also showed an enhanced tube forming inducing effect on the HUVEC. Rg1 was also demonstrated to promote angiogenesis in an in vivo Matrigel plug assay, and increase endothelial sprouting in the ex vivo rat aorta ring assay. Differential gene expression profile of HUVEC following treatment with Rg 1 revealed the expression of genes related to cell adhesion, migration and cytoskeleton, including RhoA, RhoB, IQGAP1, CALM2, Vav2 and LAMA4. Our results suggest that Rg1 can promote angiogenesis in multiple models, and this effect is partly due to the modulation of genes that are involved in the cytoskeletal dynamics, cell-cell adhesion and migration.

Original languageEnglish
Pages (from-to)205-216
Number of pages12
JournalAngiogenesis
Volume8
Issue number3
DOIs
Publication statusPublished - Dec 2005

Scopus Subject Areas

  • Physiology
  • Clinical Biochemistry
  • Cancer Research

User-Defined Keywords

  • Angiogenesis
  • Gene expression profiling
  • Ginsenosides
  • HUVEC
  • Microarray
  • Rg

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