TY - JOUR
T1 - Electroacupuncture Improves Antidepressant Effects in CUMS Rats by Protecting Hippocampal Synapse and Mitochondrion
T2 - An Ultrastructural and iTRAQ Proteomic Study
AU - Zhang, Jialing
AU - Zhang, Zhinan
AU - Zhang, Jiping
AU - Zhong, Zheng
AU - Yao, Zengyu
AU - Qu, Shanshan
AU - Huang, Yong
N1 - Funding Information:
This work was supported by the Department of Science and Technology of Guangdong Province (Grant No. 2013A032500001), Natural Science Foundation of Guangdong Province (Grant No. 2016A030313522 and 2016A030310383), National Natural Science Foundation of China (Grant No. 81603474 and 81873359), Science and Technology Program of Guangzhou (Grant No. 201707010041) and Dean's Funding Project of 2018, Nanfang Hospital, Southern Medical University (Grant No. 2018Z023), China. The authors wish to acknowledge the cooperation of the Department of Laboratory Animal Center of Southern Medical University. Meanwhile, gratitude should also be given to all those who generously contributed their time and efforts into this experiment and the paper.
Publisher Copyright:
© 2019 Jialing Zhang et al.
PY - 2019/1
Y1 - 2019/1
N2 - Electroacupuncture (EA) is considered a complementary therapy for depression. Trials also found that EA has additive benefits when combined with medication compared with medication alone. It is revealed that EA restores altered hippocampal synaptic plasticity in depressed brain. But precise molecular mechanism is poorly understood. Here, we evaluated the therapeutic effects of EA and EA combined with selective serotonin reuptake inhibitor (SSRI) on depressed (CUMS) rats. Then a new proteomics approach, isobaric tag for relative and absolute quantitation (iTRAQ), was used to explore the differential expressed synaptic protein in hippocampus between CUMS and EA-treated rats to identify the possible target molecular mechanism of its effects. We found that EA had additive benefit against depressive behaviors when combined with SSRI. Ultrastructure study on neuron showed significant change in postsynapse density (PSD) and mitochondrion. Through iTRAQ, it is found that synaptic and mitochondrial proteins were significantly changed after EA, consisting with ultrastructure study results. These findings suggest that EA improves antidepressant performance in depressed rats through protecting synaptic and mitochondrial functions in hippocampus.
AB - Electroacupuncture (EA) is considered a complementary therapy for depression. Trials also found that EA has additive benefits when combined with medication compared with medication alone. It is revealed that EA restores altered hippocampal synaptic plasticity in depressed brain. But precise molecular mechanism is poorly understood. Here, we evaluated the therapeutic effects of EA and EA combined with selective serotonin reuptake inhibitor (SSRI) on depressed (CUMS) rats. Then a new proteomics approach, isobaric tag for relative and absolute quantitation (iTRAQ), was used to explore the differential expressed synaptic protein in hippocampus between CUMS and EA-treated rats to identify the possible target molecular mechanism of its effects. We found that EA had additive benefit against depressive behaviors when combined with SSRI. Ultrastructure study on neuron showed significant change in postsynapse density (PSD) and mitochondrion. Through iTRAQ, it is found that synaptic and mitochondrial proteins were significantly changed after EA, consisting with ultrastructure study results. These findings suggest that EA improves antidepressant performance in depressed rats through protecting synaptic and mitochondrial functions in hippocampus.
UR - http://www.scopus.com/inward/record.url?scp=85065242042&partnerID=8YFLogxK
U2 - 10.1155/2019/3424698
DO - 10.1155/2019/3424698
M3 - Journal article
AN - SCOPUS:85065242042
SN - 1741-427X
VL - 2019
JO - Evidence-based Complementary and Alternative Medicine
JF - Evidence-based Complementary and Alternative Medicine
M1 - 3424698
ER -