TY - JOUR
T1 - Efficacy and Mechanism of Action of Yiru Tiaojing Granule Against Hyperprolactinemia in Vitro and in Vivo
AU - Wei, Yuanyi
AU - Wang, Xian
AU - YU, Zhiling
AU - Zhou, Wenqi
AU - Wang, Lili
AU - Qin, Fei
AU - Wang, Chunxia
AU - Hou, Lianbing
N1 - This study was supported by the Science and Technology Planning Project of Guangdong Province, China (NO. 2013A022 100 026) and the Science and Technology Planning Project of Guangzhou, China (NO. 201 508 030 004).
PY - 2015/8/7
Y1 - 2015/8/7
N2 - Yiru Tiaojing Granule, a traditional Chinese medicine formula, is used to treat hyperprolactinemia. This study was conducted to evaluate the mechanism of action and pharmacological activity of Yiru Tiaojing Granule on prolactin secretion. The animal model of hyperprolactinemia was induced by metoclopramide. The dopamine D2 receptor in hyperprolactinemia rat models was analyzed by immunohistochemistry. The biochemical parameters, including a follicle-stimulating hormone, luteinizing hormone, estradiol, progesterone, testosterone, and prolactin, were measured by an enzyme-linked immunosorbent assay. Furthermore, the expression of prolactin and the dopamine D2 receptor was analyzed by Western blotting. The components in the Yiru Tiaojing Granule-medicated serum were assayed by liquid chromatography-tandem mass spectrometry. The Yiru Tiaojing Granule significantly decreased the prolactin level in the hyperprolactinemia rat model, and increased the estradiol, luteinizing hormone, and progesterone levels. The high and medium doses of Yiru Tiaojing Granule reduced dopamine D2 receptor expression in the brain (p<0.001) and produced a similar effect on bromocriptine (p<0.001). Yiru Tiaojing Granule-medicated serum reduced (p<0.001) prolactin expression in MMQ cells in a concentration-dependent manner, but had no effects on GH3 cells. The level of the dopamine D2 receptor in MMQ cells was also increased dose-dependently (p<0.05). In addition, the protein kinase A and cyclic adenosine monophosphate in MMQ cells were significantly attenuated dose-dependently by treatment with a high and medium dose of Yiru Tiaojing Granule-medicated serum (p<0.05) and bromocriptine-medicated serum (p<0.01). The results suggested that Yiru Tiaojing Granule was effective against hyperprolactinemia, and the activation of the dopamine D2 receptor, which was related to the second messenger cyclic adenosine monophosphate and protein kinase A, might be the potential mechanism.
AB - Yiru Tiaojing Granule, a traditional Chinese medicine formula, is used to treat hyperprolactinemia. This study was conducted to evaluate the mechanism of action and pharmacological activity of Yiru Tiaojing Granule on prolactin secretion. The animal model of hyperprolactinemia was induced by metoclopramide. The dopamine D2 receptor in hyperprolactinemia rat models was analyzed by immunohistochemistry. The biochemical parameters, including a follicle-stimulating hormone, luteinizing hormone, estradiol, progesterone, testosterone, and prolactin, were measured by an enzyme-linked immunosorbent assay. Furthermore, the expression of prolactin and the dopamine D2 receptor was analyzed by Western blotting. The components in the Yiru Tiaojing Granule-medicated serum were assayed by liquid chromatography-tandem mass spectrometry. The Yiru Tiaojing Granule significantly decreased the prolactin level in the hyperprolactinemia rat model, and increased the estradiol, luteinizing hormone, and progesterone levels. The high and medium doses of Yiru Tiaojing Granule reduced dopamine D2 receptor expression in the brain (p<0.001) and produced a similar effect on bromocriptine (p<0.001). Yiru Tiaojing Granule-medicated serum reduced (p<0.001) prolactin expression in MMQ cells in a concentration-dependent manner, but had no effects on GH3 cells. The level of the dopamine D2 receptor in MMQ cells was also increased dose-dependently (p<0.05). In addition, the protein kinase A and cyclic adenosine monophosphate in MMQ cells were significantly attenuated dose-dependently by treatment with a high and medium dose of Yiru Tiaojing Granule-medicated serum (p<0.05) and bromocriptine-medicated serum (p<0.01). The results suggested that Yiru Tiaojing Granule was effective against hyperprolactinemia, and the activation of the dopamine D2 receptor, which was related to the second messenger cyclic adenosine monophosphate and protein kinase A, might be the potential mechanism.
KW - GH3 cell
KW - hyperprolactinemia
KW - mechanism
KW - MMQ cell
KW - Yiru Tiaojing Granule
UR - http://www.scopus.com/inward/record.url?scp=84942021071&partnerID=8YFLogxK
U2 - 10.1055/s-0035-1546208
DO - 10.1055/s-0035-1546208
M3 - Journal article
C2 - 26252831
AN - SCOPUS:84942021071
SN - 0032-0943
VL - 81
SP - 1255
EP - 1262
JO - Planta Medica
JF - Planta Medica
IS - 14
ER -
