Effects of supercoiling on the sequence-specific photo-modification of DNA by a vanadium(V)-peroxo complex

Daniel W.J. Kwong; O. Y. Chan; L. K. Shek; Ricky N.S. Wong

doi:10.1016/j.jinorgbio.2005.07.008

Effects of supercoiling on the sequence-specific photo-modification of DNA by a vanadium(V)-peroxo complex

Daniel W.J. Kwong^*
, O. Y. Chan
, L. K. Shek
, Ricky N.S. Wong

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

21 Citations (Scopus)

Abstract

Sequence-specific photo-modification of DNA has been demonstrated, for the first time, in a vanadium(V)-peroxo complex, NH₄[VO(O ₂)₂(5,6-Me₂phen)] (where 5,6-Me₂phen = 5,6-dimethyl-1,10-phenanthroline). Using molecular cloning technique, a consensus sequence motif of 5′-G(A/G)TA(T/C)C was identified associated with the two specific photo-modification sites, 5′-ATC and 5′-TACC found on a plasmid DNA, pBluescript, by a modified Sanger sequencing technique. DNA supercoiling was shown to be a critical prerequisite for this observed sequence-specific photo-modification activity.

Original language	English
Pages (from-to)	2062-2073
Number of pages	12
Journal	Journal of Inorganic Biochemistry
Volume	99
Issue number	10
DOIs	https://doi.org/10.1016/j.jinorgbio.2005.07.008
Publication status	Published - Oct 2005

User-Defined Keywords

DNA damage
Peroxo ligand
Sequence specific
Supercoiling
Vanadium

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10.1016/j.jinorgbio.2005.07.008

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@article{84b3dcfe2d8b4c67bca644fa594bf082,

title = "Effects of supercoiling on the sequence-specific photo-modification of DNA by a vanadium(V)-peroxo complex",

abstract = "Sequence-specific photo-modification of DNA has been demonstrated, for the first time, in a vanadium(V)-peroxo complex, NH4[VO(O 2)2(5,6-Me2phen)] (where 5,6-Me2phen = 5,6-dimethyl-1,10-phenanthroline). Using molecular cloning technique, a consensus sequence motif of 5′-G(A/G)TA(T/C)C was identified associated with the two specific photo-modification sites, 5′-ATC and 5′-TACC found on a plasmid DNA, pBluescript, by a modified Sanger sequencing technique. DNA supercoiling was shown to be a critical prerequisite for this observed sequence-specific photo-modification activity.",

keywords = "DNA damage, Peroxo ligand, Sequence specific, Supercoiling, Vanadium",

author = "Kwong, \{Daniel W.J.\} and Chan, \{O. Y.\} and Shek, \{L. K.\} and Wong, \{Ricky N.S.\}",

note = "Funding Information: This work was supported by the Hong Kong Research Grants Council (Grant No. HKBU 2055/00P). We thank Prof. S.I. Chan and Prof. C.-C. Cheng (Academia Sinica, Taipei, Taiwan) for helpful discussions.",

year = "2005",

month = oct,

doi = "10.1016/j.jinorgbio.2005.07.008",

language = "English",

volume = "99",

pages = "2062--2073",

journal = "Journal of Inorganic Biochemistry",

issn = "0162-0134",

publisher = "Elsevier Inc.",

number = "10",

}

TY - JOUR

T1 - Effects of supercoiling on the sequence-specific photo-modification of DNA by a vanadium(V)-peroxo complex

AU - Kwong, Daniel W.J.

AU - Chan, O. Y.

AU - Shek, L. K.

AU - Wong, Ricky N.S.

N1 - Funding Information: This work was supported by the Hong Kong Research Grants Council (Grant No. HKBU 2055/00P). We thank Prof. S.I. Chan and Prof. C.-C. Cheng (Academia Sinica, Taipei, Taiwan) for helpful discussions.

PY - 2005/10

Y1 - 2005/10

N2 - Sequence-specific photo-modification of DNA has been demonstrated, for the first time, in a vanadium(V)-peroxo complex, NH4[VO(O 2)2(5,6-Me2phen)] (where 5,6-Me2phen = 5,6-dimethyl-1,10-phenanthroline). Using molecular cloning technique, a consensus sequence motif of 5′-G(A/G)TA(T/C)C was identified associated with the two specific photo-modification sites, 5′-ATC and 5′-TACC found on a plasmid DNA, pBluescript, by a modified Sanger sequencing technique. DNA supercoiling was shown to be a critical prerequisite for this observed sequence-specific photo-modification activity.

AB - Sequence-specific photo-modification of DNA has been demonstrated, for the first time, in a vanadium(V)-peroxo complex, NH4[VO(O 2)2(5,6-Me2phen)] (where 5,6-Me2phen = 5,6-dimethyl-1,10-phenanthroline). Using molecular cloning technique, a consensus sequence motif of 5′-G(A/G)TA(T/C)C was identified associated with the two specific photo-modification sites, 5′-ATC and 5′-TACC found on a plasmid DNA, pBluescript, by a modified Sanger sequencing technique. DNA supercoiling was shown to be a critical prerequisite for this observed sequence-specific photo-modification activity.

KW - DNA damage

KW - Peroxo ligand

KW - Sequence specific

KW - Supercoiling

KW - Vanadium

UR - https://www.scopus.com/pages/publications/26044435985

U2 - 10.1016/j.jinorgbio.2005.07.008

DO - 10.1016/j.jinorgbio.2005.07.008

M3 - Journal article

C2 - 16112734

AN - SCOPUS:26044435985

SN - 0162-0134

VL - 99

SP - 2062

EP - 2073

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

IS - 10

ER -

Effects of supercoiling on the sequence-specific photo-modification of DNA by a vanadium(V)-peroxo complex

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