Effects of supercoiling on the sequence-specific photo-modification of DNA by a vanadium(V)-peroxo complex

Daniel W J KWONG*, O. Y. Chan, L. K. Shek, Ricky N S WONG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Sequence-specific photo-modification of DNA has been demonstrated, for the first time, in a vanadium(V)-peroxo complex, NH4[VO(O 2)2(5,6-Me2phen)] (where 5,6-Me2phen = 5,6-dimethyl-1,10-phenanthroline). Using molecular cloning technique, a consensus sequence motif of 5′-G(A/G)TA(T/C)C was identified associated with the two specific photo-modification sites, 5′-ATC and 5′-TACC found on a plasmid DNA, pBluescript, by a modified Sanger sequencing technique. DNA supercoiling was shown to be a critical prerequisite for this observed sequence-specific photo-modification activity.

Original languageEnglish
Pages (from-to)2062-2073
Number of pages12
JournalJournal of Inorganic Biochemistry
Volume99
Issue number10
DOIs
Publication statusPublished - Oct 2005

Scopus Subject Areas

  • Biochemistry
  • Inorganic Chemistry

User-Defined Keywords

  • DNA damage
  • Peroxo ligand
  • Sequence specific
  • Supercoiling
  • Vanadium

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