TY - JOUR
T1 - Effects of sulfur fumigation and heating desulfurization on quality of medicinal herbs evaluated by metabolomics and glycomics
T2 - Codonopsis Radix, a pilot study
AU - Xu, Fei
AU - Kong, Ming
AU - Xu, Jin Di
AU - Xu, Jun
AU - Jiang, Yan
AU - Li, Song Lin
N1 - Funding Information:
This study was financially supported by National Key R&D Program of China (2019YFC1711000), Natural Science Foundation of China (No. 81503245, 81872975), Hong Kong General Research Fund (12100219), and Hong Kong Baptist University (RC-start up grant).
Publisher copyright:
© 2020 Elsevier B.V. All rights reserved.
PY - 2020/11/30
Y1 - 2020/11/30
N2 - Sulfur fumigation and heating desulfurization are used together in the post-harvest processing of many medicinal herbs. However, little is known about the effects of sulfur fumigation on saccharide components, nor about the effects of heating desulfurization on all herbal constituents. In this study, metabolomics and glycomics were integrated to investigate the effects of these two processes on the chemistry of Codonopsis Radix (CR) as a pilot study. The results showed that both sulfur fumigation and heating desulfurization significantly changed the non-saccharide small-molecule metabolome and the glycome of CR in different ways. Chemical mechanisms, such as esterification, glycosidic hydrolysis, esterolysis, amide bond hydrolysis, oxidation and dehydration, are proposed to be involved. These facts strongly inspire that, in addition to investigations of how sulfur fumigation impacts non-saccharide small-molecule metabolites, researches on heating desulfurization and saccharides should be conducted so as to enable accurate, comprehensive evaluation of the quality of sulfur-fumigated herbs.
AB - Sulfur fumigation and heating desulfurization are used together in the post-harvest processing of many medicinal herbs. However, little is known about the effects of sulfur fumigation on saccharide components, nor about the effects of heating desulfurization on all herbal constituents. In this study, metabolomics and glycomics were integrated to investigate the effects of these two processes on the chemistry of Codonopsis Radix (CR) as a pilot study. The results showed that both sulfur fumigation and heating desulfurization significantly changed the non-saccharide small-molecule metabolome and the glycome of CR in different ways. Chemical mechanisms, such as esterification, glycosidic hydrolysis, esterolysis, amide bond hydrolysis, oxidation and dehydration, are proposed to be involved. These facts strongly inspire that, in addition to investigations of how sulfur fumigation impacts non-saccharide small-molecule metabolites, researches on heating desulfurization and saccharides should be conducted so as to enable accurate, comprehensive evaluation of the quality of sulfur-fumigated herbs.
KW - Codonopsis Radix
KW - Heating desulfurization
KW - Integrated metabolomics and glycomics
KW - Medicinal herb
KW - Sulfur fumigation
UR - http://www.scopus.com/inward/record.url?scp=85090116758&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2020.113581
DO - 10.1016/j.jpba.2020.113581
M3 - Journal article
C2 - 32892083
AN - SCOPUS:85090116758
SN - 0731-7085
VL - 191
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
M1 - 113581
ER -