Effects of sesquiterpenes isolated from largehead atractylodes rhizome on growth, migration, and differentiation of B16 melanoma cells

Yan Ye, Gui Xin Chou, Hui Wang, Jian Hong Chu, David W F FONG, Zhiling YU*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The aims of this study were to isolate sesquiterpene compounds from the largehead atractylodes rhizome (LAR) and to investigate their effects on B16 cancer cells. A total of 8 sesquiterpenes from LAR were identified, of which eudesm-4 (15), 7-diene-9α, 11-diol (7) was isolated for the first time. All 8 compounds inhibited growth of B16 cells, and atractylenolide I (AT-I), atractylenolide II (AT-II), and atractylenolactam (ATR) were the most potent, with IC50 values of 76.46, 84.02, and 54.88 μM, respectively. Monomer lactone or lactam structures in the 8 compounds appeared to be critical for their antiproliferative activities. In addition, AT-I, AT-II, and ATR could induce cell differentiation and inhibit cell migration. Western blot analysis indicated that 2 of the compounds, AT-I and AT-II, could inactivate ERK, where all 3 inhibited AKT activation, suggesting that Ras/ERK and PI3K/AKT signaling pathways are involved in the action mechanisms of the LAR sesquiterpene compounds.

Original languageEnglish
Pages (from-to)92-100
Number of pages9
JournalIntegrative Cancer Therapies
Volume10
Issue number1
DOIs
Publication statusPublished - Mar 2011

Scopus Subject Areas

  • Oncology
  • Complementary and alternative medicine

User-Defined Keywords

  • cell differentiation
  • cell migration
  • largehead atractylodes rhizome
  • PI3K/AKT pathway
  • Ras/ERK pathway
  • sesquiterpenes

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