Effects of midazolam on the differentiation of murine myeloid leukemia cells

Nai Ki MAK*, Y. Y. Szeto, M. C. Fung, K. N. Leung, S. K. Kwan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The effects of midazolam (MID) on the in vitro growth and differentiation of two murine myeloid leukemia WEHI 3B (JCS) and M1 cells were studied. MID inhibits the proliferation of both Ml and JCS cells in a dose-dependent manner. At the concentration of 10 μg/ml, MID was found to induce both monocytic and granulocytic differentiation of the JCS but not Ml cells. Induction of morphological differentiation of the JCS cells was also associated with the enhanced expression of the differentiation antigens Mac-1, F4/80, and Gr-1 for the cells. Results from mRNA phenotyping experiments also indicated that the expression of tumor necrosis factor (TNF-α) and neutrophil-specific J11d differentiation marker was significantly upregulated in MID-treated JCS cells. In addition, the phagocytic activity of MID-treated JCS cells was increased towards opsonized yeast cells. Results from this investigation suggested that MID may be used as an inducer for further study on the mechanisms of differentiation in these myeloid leukemia cells.

Original languageEnglish
Pages (from-to)272-281
Number of pages10
JournalChemotherapy
Volume43
Issue number4
DOIs
Publication statusPublished - 1 Jan 1997

Scopus Subject Areas

  • Oncology
  • Pharmacology
  • Drug Discovery
  • Pharmacology (medical)
  • Infectious Diseases

User-Defined Keywords

  • Differentiation
  • Leukemia inhibitory factor
  • Midazolam
  • Myeloid leukemia cells
  • Tumor necrosis factor

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