Effects of La3+ and Gd3+ on Ca2+ influx in rat hepatoma H-35 cells

Xinmin Li*, David W F FONG, Pengfei Kwok, Mengsu Yang, Patrick C.L. Wong, Jiazuan Ni

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Effects of La3+ and Gd3+ on Ca2+ influx were investigated in rat hepatoma H-35 cells by measuring the initial rate of 45Ca2+ uptake. It was found that the maximum initial rate of Ca2+ uptake was increased six- to ten-fold at low concentrations of La3+ and Gd3+. Kinetic analyses by measuring the initial rate of Ca2+ influx at different external Ca2+ concentrations indicated the existence of two intracellular exchangeable components in the basal Ca2+ system, with low and high affinities for Ca2+, and only one class of Ca2+ binding sites was observed in the La3+- or Gd3+-treated cells. For high affinity, La3+ and Gd3+ increased both kinetic parameters Km and Vmax of basal Ca2+ influx. La3+ and Gd3+ compete directly with Ca2+ for Ca2+ binding site for low affinity. The kinetics is competitive.

Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalChinese Science Bulletin
Issue number4
Publication statusPublished - Feb 1999

Scopus Subject Areas

  • General

User-Defined Keywords

  • Ca influx
  • Gd
  • La
  • Rat hepatoma H-35 cells


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