Effects of Inoculation of PAH-degrading Bacteria and Arbuscular Mycorrhizal Fungi on Responses of Ryegrass to Phenanthrene and Pyrene

Fuyong Wu, Xiezhi Yu, Shengchun Wu, Ming Hung WONG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

In order to investigate the effects of soil microorganisms on biochemical and physiological response of plants to PAHs, PAH-degrading bacteria (Acinetobacter sp.) and/or arbuscular mycorrhizal fungus (Glomus mosseae) were inoculated with ryegrass (Lolium multiflorum) under four different concentrations of phenanthrene and pyrene (0, 50 + 50, 100 + 100, 200 + 200 mg kg–1) in soils. Acinetobacter sp. played limited roles on the growth of ryegrass, chlorophyll content, water soluble carbohydrate content, malondialdehyde (MDA) content, activities of superoxide dismutase (SOD) and peroxidase (POD) in shoot. By contrast, G. mosseae significantly (P < 0.01) increased ryegrass growth, partially by improving the photosynthetic activity through increasing the chlorophyll content in shoot. G. mosseae also significantly decreased MDA content in shoot. However, G. mosseae significantly increased SOD activity in shoot, which seemed to be resulted from significantly higher pyrene concentrations in shoot. The present study suggested that AM fungi could reduce the damage of cell membranes caused by free radicals, which may be one of the mechanisms involved in mycorrhizal alleviation of plant stress under PAHs. The present study indicated that the dual inoculation was superior to single inoculation in remediating PAHs contaminated soils. Supplemental materials are available for this article. Go to the publisher's online edition of International Journal of Phytoremediation to view the supplemental file.

Original languageEnglish
Pages (from-to)109-122
Number of pages14
JournalInternational Journal of Phytoremediation
Volume16
Issue number2
DOIs
Publication statusPublished - 1 Feb 2014

Scopus Subject Areas

  • Environmental Chemistry
  • Pollution
  • Plant Science

User-Defined Keywords

  • biochemical and physiological response
  • dual inoculation
  • phytoremediation
  • toxicity of PAHs

Fingerprint

Dive into the research topics of 'Effects of Inoculation of PAH-degrading Bacteria and Arbuscular Mycorrhizal Fungi on Responses of Ryegrass to Phenanthrene and Pyrene'. Together they form a unique fingerprint.

Cite this