TY - JOUR
T1 - Effects of heat and hyposalinity on the gene expression in Acropora pruinosa larvae
AU - Chui, Apple Pui Yi
AU - Wong, Yue Him
AU - Sun, Jin
AU - Chang, Taison Ka Tai
AU - Qiu, Jian Wen
AU - Qian, Pei Yuan
AU - Ang Jr., Put
N1 - Funding Information:
This study was supported by the PEW Fellowship in Marine Conservation and the Direct Grant of the Chinese University of Hong Kong (No. 4053488) to AC, the Innovation Team Project of Universities in Guangdong Province (No. 2020KCXTD023) and the Natural Science Foundation of China (No. 42276104) to YW, and the University Grant Council (UGC) of Hong Kong General Research Fund (No. 14122215) to PA.
Publisher Copyright:
© 2023 Chui, Wong, Sun, Chang, Qiu, Qian and Ang.
PY - 2023/4/6
Y1 - 2023/4/6
N2 - Introduction: Climate change has resulted in elevated sea surface temperature as well as increased frequency of extreme weather events, e.g. cyclones and rainstorms, which could lead to reduced seawater salinity. While temperature effects on corals have been widely examined, the combined effects of both temperature and salinity on corals, especially their early stages, remain poorly known. This study aimed to examine how the larvae of Acropora pruinosa in a marginal coral habitat, Hong Kong, respond to high temperature (+5°C ambient, HT), low salinity (26 psu, LS), and the combined effects of both stressors (HTLS).Methods: We recorded larval survival and settlement success under different experimental treatments, and used RNA-Seq technique to compare the gene expression patterns of these larvae to understand the underlying molecular mechanism of stress responses.Results: Our results showed that the survivorship of coral larvae was not affected in all experimental treatments, with all larvae surviving through the 72-hour period of the experiment. Yet, larval settlement was compromised under all stress treatments. The settlement rates were 39.3%, 12%, and 0% for the elevated temperature, reduced salinity, and the combined treatment, respectively, which were all significantly lower than that under the control treatment (78%). We demonstrated that low salinity (LS) triggered responsive gene sets with functions in ATP production, protein translation, and receptor for neuroactive ligands. In addition, high temperature (HT) treatment also triggered MAPK and NF-kB signaling and apoptosis in these coral larvae. The combined stressor treatment (HTLS) acted synergistically, resulting in the up-regulation of intracellular transducers that could trigger the intrinsic apoptosis pathway. This may explain the total failure in larval settlement under HTLS that could further increase larval vulnerability in the natural environment.Discussion: Our results provide new insights into the molecular responses of coral larvae and represent an essential first step in expanding ourunderstanding of the mechanisms of tolerance that may be exhibited by coral larvae exposed to multiple stressors.
AB - Introduction: Climate change has resulted in elevated sea surface temperature as well as increased frequency of extreme weather events, e.g. cyclones and rainstorms, which could lead to reduced seawater salinity. While temperature effects on corals have been widely examined, the combined effects of both temperature and salinity on corals, especially their early stages, remain poorly known. This study aimed to examine how the larvae of Acropora pruinosa in a marginal coral habitat, Hong Kong, respond to high temperature (+5°C ambient, HT), low salinity (26 psu, LS), and the combined effects of both stressors (HTLS).Methods: We recorded larval survival and settlement success under different experimental treatments, and used RNA-Seq technique to compare the gene expression patterns of these larvae to understand the underlying molecular mechanism of stress responses.Results: Our results showed that the survivorship of coral larvae was not affected in all experimental treatments, with all larvae surviving through the 72-hour period of the experiment. Yet, larval settlement was compromised under all stress treatments. The settlement rates were 39.3%, 12%, and 0% for the elevated temperature, reduced salinity, and the combined treatment, respectively, which were all significantly lower than that under the control treatment (78%). We demonstrated that low salinity (LS) triggered responsive gene sets with functions in ATP production, protein translation, and receptor for neuroactive ligands. In addition, high temperature (HT) treatment also triggered MAPK and NF-kB signaling and apoptosis in these coral larvae. The combined stressor treatment (HTLS) acted synergistically, resulting in the up-regulation of intracellular transducers that could trigger the intrinsic apoptosis pathway. This may explain the total failure in larval settlement under HTLS that could further increase larval vulnerability in the natural environment.Discussion: Our results provide new insights into the molecular responses of coral larvae and represent an essential first step in expanding ourunderstanding of the mechanisms of tolerance that may be exhibited by coral larvae exposed to multiple stressors.
KW - Acropora pruinosa
KW - climate change
KW - coral larvae
KW - elevated seawater temperature
KW - reduced salinity
KW - transcriptomic (RNA-seq)
UR - http://www.scopus.com/inward/record.url?scp=85153506150&partnerID=8YFLogxK
U2 - 10.3389/fmars.2023.1096407
DO - 10.3389/fmars.2023.1096407
M3 - Journal article
AN - SCOPUS:85153506150
SN - 2296-7745
VL - 10
JO - Frontiers in Marine Science
JF - Frontiers in Marine Science
M1 - 1096407
ER -
