Effects of euxanthone on neuronal differentiation

Nai Ki Mak; Wen-Kui Li; Meng Zhang; Ricky N S Wong; Lai-Shan Tai; Kin Lam Yung; Hi Wun Leung

doi:10.1016/S0024-3205(99)00596-2

Effects of euxanthone on neuronal differentiation

Nai Ki Mak^*, Wen-Kui Li, Meng Zhang, Ricky N S Wong, Lai-Shan Tai, Kin Lam Yung, Hi Wun Leung

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

34 Citations (Scopus)

Abstract

The growth inhibitory and differentiation inducing effects of euxanthone (1,7-dihydroxyxanthone) from the medicinal plant Polygala caudata on the neuroblastoma (Neural 2A, subclone BU-1) were investigated. At the concentration range of 0 - 100μM, euxanthone inhibits the growth of BU-1 cells in a dose dependent manner. The 50% growth inhibitory concentration (IC₅₀) was 41μM. Significant induction of morphological differentiation and neurite growth was observed at the concentration of 100μM. Frequency of proliferative neuroblastoma cells was determined after induction of differentiation. The frequency of proliferating BU-1 cells was markedly reduced from 1/1.1 to <1/99. Confocal microscopy also confirmed that the morphological differentiation of BU-1 was associated with the expression of neurite specific marker MAP-2 protein in neurites. These data suggest that euxanthone may be one of the neuropharmacological active compounds in the medicinal plant Polygala caudata.

Original language	English
Pages (from-to)	347-354
Number of pages	8
Journal	Life Sciences
Volume	66
Issue number	4
DOIs	https://doi.org/10.1016/S0024-3205(99)00596-2
Publication status	Published - 14 Dec 1999

User-Defined Keywords

Euxanthone
Laser scanning confocal microscopy
Limiting dilution analysis
Neurite growth
Neuroblastoma

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10.1016/S0024-3205(99)00596-2

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title = "Effects of euxanthone on neuronal differentiation",

abstract = "The growth inhibitory and differentiation inducing effects of euxanthone (1,7-dihydroxyxanthone) from the medicinal plant Polygala caudata on the neuroblastoma (Neural 2A, subclone BU-1) were investigated. At the concentration range of 0 - 100μM, euxanthone inhibits the growth of BU-1 cells in a dose dependent manner. The 50% growth inhibitory concentration (IC50) was 41μM. Significant induction of morphological differentiation and neurite growth was observed at the concentration of 100μM. Frequency of proliferative neuroblastoma cells was determined after induction of differentiation. The frequency of proliferating BU-1 cells was markedly reduced from 1/1.1 to <1/99. Confocal microscopy also confirmed that the morphological differentiation of BU-1 was associated with the expression of neurite specific marker MAP-2 protein in neurites. These data suggest that euxanthone may be one of the neuropharmacological active compounds in the medicinal plant Polygala caudata.",

keywords = "Euxanthone, Laser scanning confocal microscopy, Limiting dilution analysis, Neurite growth, Neuroblastoma",

author = "Mak, {Nai Ki} and Wen-Kui Li and Meng Zhang and Wong, {Ricky N S} and Lai-Shan Tai and Yung, {Kin Lam} and Leung, {Hi Wun}",

note = "Funding Information: The authors wish to thank Mr. Yue Ying Kit and Miss Yam Wai Yin for their assistancei n technicalh elp and statisticala nalysis. The work was partly supportedb y a grant from the Industry SupportF und and the Faculty DevelopmentG rant of Hong Kong Baptist University.",

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AU - Mak, Nai Ki

AU - Li, Wen-Kui

AU - Zhang, Meng

AU - Wong, Ricky N S

AU - Tai, Lai-Shan

AU - Yung, Kin Lam

AU - Leung, Hi Wun

N1 - Funding Information: The authors wish to thank Mr. Yue Ying Kit and Miss Yam Wai Yin for their assistancei n technicalh elp and statisticala nalysis. The work was partly supportedb y a grant from the Industry SupportF und and the Faculty DevelopmentG rant of Hong Kong Baptist University.

PY - 1999/12/14

Y1 - 1999/12/14

N2 - The growth inhibitory and differentiation inducing effects of euxanthone (1,7-dihydroxyxanthone) from the medicinal plant Polygala caudata on the neuroblastoma (Neural 2A, subclone BU-1) were investigated. At the concentration range of 0 - 100μM, euxanthone inhibits the growth of BU-1 cells in a dose dependent manner. The 50% growth inhibitory concentration (IC50) was 41μM. Significant induction of morphological differentiation and neurite growth was observed at the concentration of 100μM. Frequency of proliferative neuroblastoma cells was determined after induction of differentiation. The frequency of proliferating BU-1 cells was markedly reduced from 1/1.1 to <1/99. Confocal microscopy also confirmed that the morphological differentiation of BU-1 was associated with the expression of neurite specific marker MAP-2 protein in neurites. These data suggest that euxanthone may be one of the neuropharmacological active compounds in the medicinal plant Polygala caudata.

AB - The growth inhibitory and differentiation inducing effects of euxanthone (1,7-dihydroxyxanthone) from the medicinal plant Polygala caudata on the neuroblastoma (Neural 2A, subclone BU-1) were investigated. At the concentration range of 0 - 100μM, euxanthone inhibits the growth of BU-1 cells in a dose dependent manner. The 50% growth inhibitory concentration (IC50) was 41μM. Significant induction of morphological differentiation and neurite growth was observed at the concentration of 100μM. Frequency of proliferative neuroblastoma cells was determined after induction of differentiation. The frequency of proliferating BU-1 cells was markedly reduced from 1/1.1 to <1/99. Confocal microscopy also confirmed that the morphological differentiation of BU-1 was associated with the expression of neurite specific marker MAP-2 protein in neurites. These data suggest that euxanthone may be one of the neuropharmacological active compounds in the medicinal plant Polygala caudata.

KW - Euxanthone

KW - Laser scanning confocal microscopy

KW - Limiting dilution analysis

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KW - Neuroblastoma

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DO - 10.1016/S0024-3205(99)00596-2

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JO - Life Sciences

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Effects of euxanthone on neuronal differentiation

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