TY - JOUR
T1 - Effects of curcumin in treatment of experimental pulmonary fibrosis
T2 - A comparison with hydrocortisone
AU - XU, Min
AU - Deng, Bin
AU - Chow, Yeuk lung
AU - ZHAO, Zhongzhen
AU - Hu, Bin
N1 - Funding Information:
This project was supported by a grant (FRG/03-04/II-35) from Hong Kong Baptist University. The authors would like to thank Dr. Mengjun Hou, Mr. Michael Wong and Ms. Irene Koo for their help in this study.
PY - 2007/6/13
Y1 - 2007/6/13
N2 - To compare curcumin with hydrocortisone for treating bleomycin-induced pulmonary fibrosis (BLMPF), four groups of rats were injected with 1.5 mg/kg bleomycin intratracheally. Then the Group HC rats were treated with three injections of 2 mg/kg hydrocortisone i.p.; Group CH and CL rats, respectively, were orally given 500 or 250 mg/kg curcumin daily; and Group PC rats were given deionized water alone. After 28 days of treatment, lung samples were examined by H-E staining, Masson's staining and immunohistochemical analyses and pulmonary type I collagen (Col-I), inducible nitric oxide synthetase (iNOS) and transforming growth factor-β1 (TGF-β1) were determined by Western blotting and real-time RT PCR analyses. The results showed that (1) Group PC rats had histopathological characteristics of BLMPF with significant increase in their protein/mRNA expressions of Col-I (+114%/+173%), iNOS (+146%/+523%) and TGF-β1 (+476%/+527%) (P < 0.01); (2) in Group HC, CH and CL rats, protein/mRNA expressions of Col-I (-39%/-52%, -31%/-57%, -33%/-58%), iNOS (-31%/-51%, -31%/-79%, -31%/-47%) and TGF-β1 (-64%/-78%, -75%/-74%, -81%/-79%) were significantly lower than Group PC (P < 0.05); (3) except for levels of TGF-β1 protein, there was no significant difference among Group CH, CL and HC rats (P > 0.05). It suggests that curcumin may play a similar role as hydrocortisone in preventing BLMPF.
AB - To compare curcumin with hydrocortisone for treating bleomycin-induced pulmonary fibrosis (BLMPF), four groups of rats were injected with 1.5 mg/kg bleomycin intratracheally. Then the Group HC rats were treated with three injections of 2 mg/kg hydrocortisone i.p.; Group CH and CL rats, respectively, were orally given 500 or 250 mg/kg curcumin daily; and Group PC rats were given deionized water alone. After 28 days of treatment, lung samples were examined by H-E staining, Masson's staining and immunohistochemical analyses and pulmonary type I collagen (Col-I), inducible nitric oxide synthetase (iNOS) and transforming growth factor-β1 (TGF-β1) were determined by Western blotting and real-time RT PCR analyses. The results showed that (1) Group PC rats had histopathological characteristics of BLMPF with significant increase in their protein/mRNA expressions of Col-I (+114%/+173%), iNOS (+146%/+523%) and TGF-β1 (+476%/+527%) (P < 0.01); (2) in Group HC, CH and CL rats, protein/mRNA expressions of Col-I (-39%/-52%, -31%/-57%, -33%/-58%), iNOS (-31%/-51%, -31%/-79%, -31%/-47%) and TGF-β1 (-64%/-78%, -75%/-74%, -81%/-79%) were significantly lower than Group PC (P < 0.05); (3) except for levels of TGF-β1 protein, there was no significant difference among Group CH, CL and HC rats (P > 0.05). It suggests that curcumin may play a similar role as hydrocortisone in preventing BLMPF.
KW - Bleomycin
KW - Curcumin
KW - Hydrocortisone
KW - Pulmonary fibrosis
UR - http://www.scopus.com/inward/record.url?scp=34248577489&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2007.03.011
DO - 10.1016/j.jep.2007.03.011
M3 - Journal article
C2 - 17434272
AN - SCOPUS:34248577489
SN - 0378-8741
VL - 112
SP - 292
EP - 299
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
IS - 2
ER -