TY - JOUR
T1 - Effects of Cinobufagin on the Proliferation, Migration, and Invasion of H1299 Lung Cancer Cells
AU - Sun, Mingna
AU - Huang, Dongyu
AU - Liu, Yun
AU - Chen, Haifang
AU - Yu, Hua
AU - Zhang, Guobin
AU - Chen, Qilei
AU - Chen, Hubiao
AU - Zhang, Jianye
N1 - Funding Information:
This work was supported by the National Key R&D Program of China (2021YFE0202000), National Natural Science Foundation of China (U1903126), Fund of Guangdong Education Department (2021ZDZX2006), Traditional Chinese Medicine Bureau of Guangdong Province (20222121), Discipline Project of the 14th Five‐year Plan of Guangzhou Medical University (06‐410‐2107218), and the Innovation and Technology Fund in Hong Kong (PRP/036/20FX; MHP/023/20).
Publisher Copyright:
© 2022 Wiley-VHCA AG, Zurich, Switzerland.
PY - 2023/1
Y1 - 2023/1
N2 - Cinobufagin (CB), with its steroidal nucleus structure, is one of the major, biologically active components of Chan Su. Recent studies have shown that CB exerts inhibitory effects against numerous cancer cells. However, the effects of CB regarding the metastasis of non-small cell lung cancer (NSCLC) and the involved mechanisms need to be further studied. The purpose of the present study aimed to report the inhibitory function of CB against proliferation and metastasis of H1299 cells. CB inhibited proliferation of H1299 lung cancer cells with an IC50 value of 0.035±0.008 μM according to the results of MTT assays. Antiproliferative activity was also observed in colony forming cell assays. In addition, 5-ethynyl-2’-deoxyuridine (EdU) retention assays revealed that CB significantly inhibited the rate of DNA synthesis in H1299 cells. Moreover, results of the scratch wound healing assays and transwell migration assays displayed that CB exhibited significant inhibition against migration and invasion of H1299 cells. Furthermore, CB could concentration-dependently reduce the expression of integrin α2, β-catenin, FAK, Src, c-Myc, and STAT3 in H1299 cells. These western blotting results indicated that CB might target integrin α2, β-catenin, FAK and Src to suppress invasion and migration of NSCLC, which was consistent with the network pharmacology analysis results. Collectively, findings of the current study suggest that CB possesses promising activity against NSCLC growth and metastasis.
AB - Cinobufagin (CB), with its steroidal nucleus structure, is one of the major, biologically active components of Chan Su. Recent studies have shown that CB exerts inhibitory effects against numerous cancer cells. However, the effects of CB regarding the metastasis of non-small cell lung cancer (NSCLC) and the involved mechanisms need to be further studied. The purpose of the present study aimed to report the inhibitory function of CB against proliferation and metastasis of H1299 cells. CB inhibited proliferation of H1299 lung cancer cells with an IC50 value of 0.035±0.008 μM according to the results of MTT assays. Antiproliferative activity was also observed in colony forming cell assays. In addition, 5-ethynyl-2’-deoxyuridine (EdU) retention assays revealed that CB significantly inhibited the rate of DNA synthesis in H1299 cells. Moreover, results of the scratch wound healing assays and transwell migration assays displayed that CB exhibited significant inhibition against migration and invasion of H1299 cells. Furthermore, CB could concentration-dependently reduce the expression of integrin α2, β-catenin, FAK, Src, c-Myc, and STAT3 in H1299 cells. These western blotting results indicated that CB might target integrin α2, β-catenin, FAK and Src to suppress invasion and migration of NSCLC, which was consistent with the network pharmacology analysis results. Collectively, findings of the current study suggest that CB possesses promising activity against NSCLC growth and metastasis.
KW - cinobufagin
KW - H1299 cell
KW - lung cancer
KW - metastasis
KW - proliferation
UR - http://www.scopus.com/inward/record.url?scp=85145552049&partnerID=8YFLogxK
U2 - 10.1002/cbdv.202200961
DO - 10.1002/cbdv.202200961
M3 - Journal article
SN - 1612-1872
VL - 20
JO - Chemistry and Biodiversity
JF - Chemistry and Biodiversity
IS - 1
M1 - e202200961
ER -