Effects of Ambient Atmospheric PM                         2.5                         , 1-Nitropyrene and 9-Nitroanthracene on DNA Damage and Oxidative Stress in Hearts of Rats

Lifang Zhao; Li Zhang; Minghui Chen; Chuan Dong; Ruijin Li; Zongwei CAI

doi:10.1007/s12012-018-9488-5

Effects of Ambient Atmospheric PM _2.5 , 1-Nitropyrene and 9-Nitroanthracene on DNA Damage and Oxidative Stress in Hearts of Rats

Lifang Zhao
, Li Zhang
, Minghui Chen
, Chuan Dong
, Ruijin Li^*
, Zongwei CAI

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

27 Citations (Scopus)

Abstract

Exposure to fine particulate matter (PM _2.5 ) increased the risks of cardiovascular diseases. PM _2.5 -bound 1-nitropyrene (1-NP) and 9-nitroanthracene (9-NA) are released from the incomplete combustion of fossil fuels and derived from polycyclic aromatic hydrocarbons (PAHs). The toxicities of 1-NP and 9-NA are mainly reflected in their carcinogenicity and mutagenicity. However, studies of PM _2.5 -bound 1-NP and 9-NA on the cardiac genotoxicity are limited so far. In this study, histopathology, DNA damage, DNA repair-related gene expression, and oxidative stress were investigated in the hearts of male Wistar rats exposed to PM _2.5 [1.5 mg/kg body weight (b.w.)] or three different dosages of 1-NP (1.0 × 10 ^{− 5} , 4.0 × 10 ^{− 5} , and 1.6 × 10 ^{− 4} mg/kg b.w.) or 9-NA (1.3 × 10 ^{− 5} , 4.0 × 10 ^{− 5} , and 1.2 × 10 ^{− 4} mg/kg b.w.). The results revealed that (1) PM _2.5 , higher dosages of 1-NP (4.0 × 10 ^{− 5} and 1.6 × 10 ^{− 4} mg/kg b.w.) and 9-NA (4.0 × 10 ^{− 5} and 1.2 × 10 ^{− 4} mg/kg b.w.) caused obvious pathological responses and DNA damage (DNA strand breaks, 8-OHdG formation and DNA–protein cross-link), accompanied by increasing OGG1 and GADD153 expression while inhibiting MTH1 and XRCC1 expression in rat hearts. Also, they elevated the hemeoxygenase-1 (HO-1), glutathione S-transferase (GST), and malondialdehyde (MDA) levels and decreased superoxide dismutase (SOD) activity compared with the control. (2) The lowest dosages 1-NP or 9-NA could not cause DNA damage and oxidative stress. (3) At the approximately equivalent dose level, PM _2.5 -induced DNA damage effects were more obvious than 1-NP or 9-NA along with positive correlation. Taken together, heart DNA damage caused by PM _2.5 , 1-NP and 9-NA may be mediated partially through influencing the DNA repair capacity and causing oxidative stress, and such negative effects might be related to the genotoxicity PM _2.5 , 1-NP, and 9-NA.

Original language	English
Pages (from-to)	178-190
Number of pages	13
Journal	Cardiovascular Toxicology
Volume	19
Issue number	2
DOIs	https://doi.org/10.1007/s12012-018-9488-5
Publication status	Published - 15 Apr 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

1-Nitropyrene
9-Nitroanthracene
DNA damage
Fine particulate matter
Oxidative stress
Rat heart

Access to Document

10.1007/s12012-018-9488-5

Cite this

@article{d0951db1e2404281a049fbe210880f14,

title = "Effects of Ambient Atmospheric PM 2.5 , 1-Nitropyrene and 9-Nitroanthracene on DNA Damage and Oxidative Stress in Hearts of Rats",

abstract = " Exposure to fine particulate matter (PM 2.5 ) increased the risks of cardiovascular diseases. PM 2.5 -bound 1-nitropyrene (1-NP) and 9-nitroanthracene (9-NA) are released from the incomplete combustion of fossil fuels and derived from polycyclic aromatic hydrocarbons (PAHs). The toxicities of 1-NP and 9-NA are mainly reflected in their carcinogenicity and mutagenicity. However, studies of PM 2.5 -bound 1-NP and 9-NA on the cardiac genotoxicity are limited so far. In this study, histopathology, DNA damage, DNA repair-related gene expression, and oxidative stress were investigated in the hearts of male Wistar rats exposed to PM 2.5 [1.5 mg/kg body weight (b.w.)] or three different dosages of 1-NP (1.0 × 10 − 5 , 4.0 × 10 − 5 , and 1.6 × 10 − 4 mg/kg b.w.) or 9-NA (1.3 × 10 − 5 , 4.0 × 10 − 5 , and 1.2 × 10 − 4 mg/kg b.w.). The results revealed that (1) PM 2.5 , higher dosages of 1-NP (4.0 × 10 − 5 and 1.6 × 10 − 4 mg/kg b.w.) and 9-NA (4.0 × 10 − 5 and 1.2 × 10 − 4 mg/kg b.w.) caused obvious pathological responses and DNA damage (DNA strand breaks, 8-OHdG formation and DNA–protein cross-link), accompanied by increasing OGG1 and GADD153 expression while inhibiting MTH1 and XRCC1 expression in rat hearts. Also, they elevated the hemeoxygenase-1 (HO-1), glutathione S-transferase (GST), and malondialdehyde (MDA) levels and decreased superoxide dismutase (SOD) activity compared with the control. (2) The lowest dosages 1-NP or 9-NA could not cause DNA damage and oxidative stress. (3) At the approximately equivalent dose level, PM 2.5 -induced DNA damage effects were more obvious than 1-NP or 9-NA along with positive correlation. Taken together, heart DNA damage caused by PM 2.5 , 1-NP and 9-NA may be mediated partially through influencing the DNA repair capacity and causing oxidative stress, and such negative effects might be related to the genotoxicity PM 2.5 , 1-NP, and 9-NA.",

keywords = "1-Nitropyrene, 9-Nitroanthracene, DNA damage, Fine particulate matter, Oxidative stress, Rat heart",

author = "Lifang Zhao and Li Zhang and Minghui Chen and Chuan Dong and Ruijin Li and Zongwei CAI",

note = "Funding Information: Acknowledgements This research was supported by the National Natural Science Foundation of China (No. 91543202) and the Nature Science Foundation of Shanxi Province in China (No. 2014011036-2).",

year = "2019",

month = apr,

day = "15",

doi = "10.1007/s12012-018-9488-5",

language = "English",

volume = "19",

pages = "178--190",

journal = "Cardiovascular Toxicology",

issn = "1530-7905",

publisher = "Springer",

number = "2",

}

Effects of Ambient Atmospheric PM _2.5 , 1-Nitropyrene and 9-Nitroanthracene on DNA Damage and Oxidative Stress in Hearts of Rats. / Zhao, Lifang; Zhang, Li; Chen, Minghui et al.
In: Cardiovascular Toxicology, Vol. 19, No. 2, 15.04.2019, p. 178-190.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - Effects of Ambient Atmospheric PM 2.5 , 1-Nitropyrene and 9-Nitroanthracene on DNA Damage and Oxidative Stress in Hearts of Rats

AU - Zhao, Lifang

AU - Zhang, Li

AU - Chen, Minghui

AU - Dong, Chuan

AU - Li, Ruijin

AU - CAI, Zongwei

N1 - Funding Information: Acknowledgements This research was supported by the National Natural Science Foundation of China (No. 91543202) and the Nature Science Foundation of Shanxi Province in China (No. 2014011036-2).

PY - 2019/4/15

Y1 - 2019/4/15

N2 - Exposure to fine particulate matter (PM 2.5 ) increased the risks of cardiovascular diseases. PM 2.5 -bound 1-nitropyrene (1-NP) and 9-nitroanthracene (9-NA) are released from the incomplete combustion of fossil fuels and derived from polycyclic aromatic hydrocarbons (PAHs). The toxicities of 1-NP and 9-NA are mainly reflected in their carcinogenicity and mutagenicity. However, studies of PM 2.5 -bound 1-NP and 9-NA on the cardiac genotoxicity are limited so far. In this study, histopathology, DNA damage, DNA repair-related gene expression, and oxidative stress were investigated in the hearts of male Wistar rats exposed to PM 2.5 [1.5 mg/kg body weight (b.w.)] or three different dosages of 1-NP (1.0 × 10 − 5 , 4.0 × 10 − 5 , and 1.6 × 10 − 4 mg/kg b.w.) or 9-NA (1.3 × 10 − 5 , 4.0 × 10 − 5 , and 1.2 × 10 − 4 mg/kg b.w.). The results revealed that (1) PM 2.5 , higher dosages of 1-NP (4.0 × 10 − 5 and 1.6 × 10 − 4 mg/kg b.w.) and 9-NA (4.0 × 10 − 5 and 1.2 × 10 − 4 mg/kg b.w.) caused obvious pathological responses and DNA damage (DNA strand breaks, 8-OHdG formation and DNA–protein cross-link), accompanied by increasing OGG1 and GADD153 expression while inhibiting MTH1 and XRCC1 expression in rat hearts. Also, they elevated the hemeoxygenase-1 (HO-1), glutathione S-transferase (GST), and malondialdehyde (MDA) levels and decreased superoxide dismutase (SOD) activity compared with the control. (2) The lowest dosages 1-NP or 9-NA could not cause DNA damage and oxidative stress. (3) At the approximately equivalent dose level, PM 2.5 -induced DNA damage effects were more obvious than 1-NP or 9-NA along with positive correlation. Taken together, heart DNA damage caused by PM 2.5 , 1-NP and 9-NA may be mediated partially through influencing the DNA repair capacity and causing oxidative stress, and such negative effects might be related to the genotoxicity PM 2.5 , 1-NP, and 9-NA.

AB - Exposure to fine particulate matter (PM 2.5 ) increased the risks of cardiovascular diseases. PM 2.5 -bound 1-nitropyrene (1-NP) and 9-nitroanthracene (9-NA) are released from the incomplete combustion of fossil fuels and derived from polycyclic aromatic hydrocarbons (PAHs). The toxicities of 1-NP and 9-NA are mainly reflected in their carcinogenicity and mutagenicity. However, studies of PM 2.5 -bound 1-NP and 9-NA on the cardiac genotoxicity are limited so far. In this study, histopathology, DNA damage, DNA repair-related gene expression, and oxidative stress were investigated in the hearts of male Wistar rats exposed to PM 2.5 [1.5 mg/kg body weight (b.w.)] or three different dosages of 1-NP (1.0 × 10 − 5 , 4.0 × 10 − 5 , and 1.6 × 10 − 4 mg/kg b.w.) or 9-NA (1.3 × 10 − 5 , 4.0 × 10 − 5 , and 1.2 × 10 − 4 mg/kg b.w.). The results revealed that (1) PM 2.5 , higher dosages of 1-NP (4.0 × 10 − 5 and 1.6 × 10 − 4 mg/kg b.w.) and 9-NA (4.0 × 10 − 5 and 1.2 × 10 − 4 mg/kg b.w.) caused obvious pathological responses and DNA damage (DNA strand breaks, 8-OHdG formation and DNA–protein cross-link), accompanied by increasing OGG1 and GADD153 expression while inhibiting MTH1 and XRCC1 expression in rat hearts. Also, they elevated the hemeoxygenase-1 (HO-1), glutathione S-transferase (GST), and malondialdehyde (MDA) levels and decreased superoxide dismutase (SOD) activity compared with the control. (2) The lowest dosages 1-NP or 9-NA could not cause DNA damage and oxidative stress. (3) At the approximately equivalent dose level, PM 2.5 -induced DNA damage effects were more obvious than 1-NP or 9-NA along with positive correlation. Taken together, heart DNA damage caused by PM 2.5 , 1-NP and 9-NA may be mediated partially through influencing the DNA repair capacity and causing oxidative stress, and such negative effects might be related to the genotoxicity PM 2.5 , 1-NP, and 9-NA.

KW - 1-Nitropyrene

KW - 9-Nitroanthracene

KW - DNA damage

KW - Fine particulate matter

KW - Oxidative stress

KW - Rat heart

UR - https://www.scopus.com/pages/publications/85056580867

U2 - 10.1007/s12012-018-9488-5

DO - 10.1007/s12012-018-9488-5

M3 - Journal article

C2 - 30421179

AN - SCOPUS:85056580867

SN - 1530-7905

VL - 19

SP - 178

EP - 190

JO - Cardiovascular Toxicology

JF - Cardiovascular Toxicology

IS - 2

ER -