TY - JOUR
T1 - Effective photocatalytic inactivation of the plant-pathogen Rhizobium radiobacter by carbon-based material
T2 - Mechanism and agriculture application
AU - Xiao, Kemeng
AU - Liu, Tieyuan
AU - Yin, Panqing
AU - Ren, Xiaoning
AU - Liang, Jun
AU - Zhan, Wugen
AU - Zhang, Jianhua
AU - Wang, Bo
AU - Wong, Po Keung
N1 - Funding Information:
The author thanks to the funding support from CAS Key Laboratory of Quantitative Engineering Biology , Shenzhen Institute of Synthetic Biology , Shenzhen Institutes of Advanced Technology , Chinese Academy of Sciences . The author also thanks to the support of Ph.D Research Start-up Foundation of Northwest AF University (2452019150). The research project was also supported by Technology and Business Development Fund, The Chinese University of Hong Kong (No. TBF18SCI006 ).
Funding Information:
The author thanks to the funding support from CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences. The author also thanks to the support of Ph.D Research Start-up Foundation of Northwest AF University (2452019150). The research project was also supported by Technology and Business Development Fund, The Chinese University of Hong Kong (No. TBF18SCI006).
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/3/1
Y1 - 2021/3/1
N2 - Photocatalysts made of earth-abundant elements using simple fabrication methods are highly desirable for bacterial inactivation in practical applications. This study proved that the carbon-based g-C3N4 could act as an effective antagonist to the ubiquitous plant-pathogen Rhizobium radiobacter with good photostability and reusability under visible light. The applying of g-C3N4 efficiently improved the survivability of tobacco seedlings under the stress of R. radiobacter and showed no adverse effect on tobacco growth compared with the common metal-based TiO2 and CdS photocatalysts. The systematic mechanism studies revealed that the photoinduced reactive species (RSs) were strongly involved in the photocatalytic bacterial inactivation process, with an effectiveness of h+ >O2− >OH > H2O2 > e− > 1O2. The direct contact between g-C3N4 and bacterial cells was also essential for the effective bacterial inactivation. Although bacterial self-protection system (SOD and CAT enzymes) functioned in the initial period, the accumulated RSs damaged cell membrane structure as well as membrane-associated respiration and ATP synthesis ability, finally leading to the leakage of cellular building blocks (K+, TOC, DNA and RNA) and irreversible cell death. This study illustrated that the g-C3N4 could be used as an ideal photocatalytic bactericide towards agriculture application to improve the plant resistance to pathogens with good biocompatibility and low-cost.
AB - Photocatalysts made of earth-abundant elements using simple fabrication methods are highly desirable for bacterial inactivation in practical applications. This study proved that the carbon-based g-C3N4 could act as an effective antagonist to the ubiquitous plant-pathogen Rhizobium radiobacter with good photostability and reusability under visible light. The applying of g-C3N4 efficiently improved the survivability of tobacco seedlings under the stress of R. radiobacter and showed no adverse effect on tobacco growth compared with the common metal-based TiO2 and CdS photocatalysts. The systematic mechanism studies revealed that the photoinduced reactive species (RSs) were strongly involved in the photocatalytic bacterial inactivation process, with an effectiveness of h+ >O2− >OH > H2O2 > e− > 1O2. The direct contact between g-C3N4 and bacterial cells was also essential for the effective bacterial inactivation. Although bacterial self-protection system (SOD and CAT enzymes) functioned in the initial period, the accumulated RSs damaged cell membrane structure as well as membrane-associated respiration and ATP synthesis ability, finally leading to the leakage of cellular building blocks (K+, TOC, DNA and RNA) and irreversible cell death. This study illustrated that the g-C3N4 could be used as an ideal photocatalytic bactericide towards agriculture application to improve the plant resistance to pathogens with good biocompatibility and low-cost.
KW - Bacterial inactivation
KW - Graphitic carbon nitride
KW - Mechanism
KW - Plant-pathogen
KW - Visible-light-driven
UR - http://www.scopus.com/inward/record.url?scp=85096932108&partnerID=8YFLogxK
U2 - 10.1016/j.cej.2020.127047
DO - 10.1016/j.cej.2020.127047
M3 - Journal article
AN - SCOPUS:85096932108
SN - 1385-8947
VL - 407
JO - Chemical Engineering Journal
JF - Chemical Engineering Journal
M1 - 127047
ER -