TY - JOUR
T1 - Effect of Tanshinone IIA on the Noncovalent Interaction Between Warfarin and Human Serum Albumin Studied by Electrospray Ionization Mass Spectrometry
AU - Liu, Jie
AU - Wang, Xiaoru
AU - CAI, Zongwei
AU - Lee, Frank S.C.
N1 - Funding Information:
The authors acknowledge financial support for this study from Qingdao Biomedical Research and Testing Center, Laoshan Scientific Department (LS-05-KJZX-76) and the Research Grant Council, University Grants Committee of Hong Kong (HKBU2014/05M).
PY - 2008/10
Y1 - 2008/10
N2 - Enhanced anticoagulation and/or even bleeding are often observed when patients on long-term warfarin (WAR) therapy consumed Danshen, a well-known medicinal herb in traditional Chinese medicine (TCM). This study demonstrates that altered WAR metabolism, arising from its interaction with the active components in Danshen, played a significant role in this curative effect. Mass spectrometric techniques including ESI-ITMS (electrospray ionization ion-trap mass spectrometry) and ESI-TOF (time-of-flight)-MS have been developed for the study of such drug-herb interactions. The experimental approach involved a detailed analysis and comparison of WAR metabolites in vivo from blood or urine of rats that had been orally administrated with WAR, either singly or together with the representative bioactive component of Danshen-lipid soluble TIIA (Tanshinon IIA), and a study of the interaction of human serum albumin (HSA), WAR, and water-soluble sodium tanshinone IIA sulfonate (STS) in vitro. Results demonstrate that TIIA accelerates the metabolic rate of WAR, whereas STS displaces WAR from the WAR-HSA complex, resulting in an increase of free WAR concentration in blood. It is suggested that the elevated level and enhanced metabolism of WAR is responsible for the over-anticoagulation effect observed.
AB - Enhanced anticoagulation and/or even bleeding are often observed when patients on long-term warfarin (WAR) therapy consumed Danshen, a well-known medicinal herb in traditional Chinese medicine (TCM). This study demonstrates that altered WAR metabolism, arising from its interaction with the active components in Danshen, played a significant role in this curative effect. Mass spectrometric techniques including ESI-ITMS (electrospray ionization ion-trap mass spectrometry) and ESI-TOF (time-of-flight)-MS have been developed for the study of such drug-herb interactions. The experimental approach involved a detailed analysis and comparison of WAR metabolites in vivo from blood or urine of rats that had been orally administrated with WAR, either singly or together with the representative bioactive component of Danshen-lipid soluble TIIA (Tanshinon IIA), and a study of the interaction of human serum albumin (HSA), WAR, and water-soluble sodium tanshinone IIA sulfonate (STS) in vitro. Results demonstrate that TIIA accelerates the metabolic rate of WAR, whereas STS displaces WAR from the WAR-HSA complex, resulting in an increase of free WAR concentration in blood. It is suggested that the elevated level and enhanced metabolism of WAR is responsible for the over-anticoagulation effect observed.
UR - http://www.scopus.com/inward/record.url?scp=53349129384&partnerID=8YFLogxK
U2 - 10.1016/j.jasms.2008.06.005
DO - 10.1016/j.jasms.2008.06.005
M3 - Journal article
C2 - 18657993
AN - SCOPUS:53349129384
SN - 1044-0305
VL - 19
SP - 1568
EP - 1575
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 10
ER -
