Effect of sinomenine on gene expression of the IL-1β-activated human synovial sarcoma

X. J. Li; Patrick Y K YUE; W. Y. Ha; Daisy Y.L. Wong; Mandy M.Y. Tin; P. X. Wang; Ricky N S WONG; L. Liu

doi:10.1016/j.lfs.2006.02.014

Effect of sinomenine on gene expression of the IL-1β-activated human synovial sarcoma

X. J. Li, Patrick Y K YUE, W. Y. Ha, Daisy Y.L. Wong, Mandy M.Y. Tin, P. X. Wang, Ricky N S WONG, L. Liu^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

44 Citations (Scopus)

Abstract

Sinomenine is an alkaloid with pharmacological effects of anti-inflammation, anti-angiogenesis, anti-arthritis and immunosuppression. This study aimed to investigate the effect of sinomenine on gene expression of human synovial sarcoma cells (Hs701.T) activated by IL-1β. The proliferative effect of sinomenine was examined in the presence or absence of IL-1β by the [³H]-thymidine incorporation and MTT assay, respectively. Using DNA microarray technology and RT-PCR, the activating action of IL-1β and modulatory effect of sinomenine on Hs701.T were simultaneously determined. Results showed that IL-1β could stimulate the proliferation and gene expression of Hs701.T cells. Sinomenine could significantly inhibit proliferation of IL-1β-activated Hs701.T cells and suppress expression of 17 genes including IL-6, PlGF, Daxx, and HSP27. These genes were found to be important in tumor progression through the mediation of inflammation, cell adhesion, proliferation, apoptosis and angiogenesis. In conclusion, our study provides supplementary information for the further studies on the pharmacological effects of sinomenine acting on synovial sarcoma.

Original language	English
Pages (from-to)	665-673
Number of pages	9
Journal	Life Sciences
Volume	79
Issue number	7
DOIs	https://doi.org/10.1016/j.lfs.2006.02.014
Publication status	Published - 10 Jul 2006

Scopus Subject Areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

User-Defined Keywords

DNA microarray
Gene expression
Hs701.T
IL-1β
Sinomenine
Synovial sarcoma

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10.1016/j.lfs.2006.02.014

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title = "Effect of sinomenine on gene expression of the IL-1β-activated human synovial sarcoma",

abstract = "Sinomenine is an alkaloid with pharmacological effects of anti-inflammation, anti-angiogenesis, anti-arthritis and immunosuppression. This study aimed to investigate the effect of sinomenine on gene expression of human synovial sarcoma cells (Hs701.T) activated by IL-1β. The proliferative effect of sinomenine was examined in the presence or absence of IL-1β by the [3H]-thymidine incorporation and MTT assay, respectively. Using DNA microarray technology and RT-PCR, the activating action of IL-1β and modulatory effect of sinomenine on Hs701.T were simultaneously determined. Results showed that IL-1β could stimulate the proliferation and gene expression of Hs701.T cells. Sinomenine could significantly inhibit proliferation of IL-1β-activated Hs701.T cells and suppress expression of 17 genes including IL-6, PlGF, Daxx, and HSP27. These genes were found to be important in tumor progression through the mediation of inflammation, cell adhesion, proliferation, apoptosis and angiogenesis. In conclusion, our study provides supplementary information for the further studies on the pharmacological effects of sinomenine acting on synovial sarcoma.",

keywords = "DNA microarray, Gene expression, Hs701.T, IL-1β, Sinomenine, Synovial sarcoma",

author = "Li, {X. J.} and YUE, {Patrick Y K} and Ha, {W. Y.} and Wong, {Daisy Y.L.} and Tin, {Mandy M.Y.} and Wang, {P. X.} and WONG, {Ricky N S} and L. Liu",

note = "Funding Information: This work was supported by the Earmark Research Grants (RGC Ref no. HKBU 2144/03M) of the Research Grant Council, Hong Kong SAR Government.",

year = "2006",

month = jul,

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doi = "10.1016/j.lfs.2006.02.014",

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journal = "Life Sciences",

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TY - JOUR

T1 - Effect of sinomenine on gene expression of the IL-1β-activated human synovial sarcoma

AU - Li, X. J.

AU - YUE, Patrick Y K

AU - Ha, W. Y.

AU - Wong, Daisy Y.L.

AU - Tin, Mandy M.Y.

AU - Wang, P. X.

AU - WONG, Ricky N S

AU - Liu, L.

N1 - Funding Information: This work was supported by the Earmark Research Grants (RGC Ref no. HKBU 2144/03M) of the Research Grant Council, Hong Kong SAR Government.

PY - 2006/7/10

Y1 - 2006/7/10

N2 - Sinomenine is an alkaloid with pharmacological effects of anti-inflammation, anti-angiogenesis, anti-arthritis and immunosuppression. This study aimed to investigate the effect of sinomenine on gene expression of human synovial sarcoma cells (Hs701.T) activated by IL-1β. The proliferative effect of sinomenine was examined in the presence or absence of IL-1β by the [3H]-thymidine incorporation and MTT assay, respectively. Using DNA microarray technology and RT-PCR, the activating action of IL-1β and modulatory effect of sinomenine on Hs701.T were simultaneously determined. Results showed that IL-1β could stimulate the proliferation and gene expression of Hs701.T cells. Sinomenine could significantly inhibit proliferation of IL-1β-activated Hs701.T cells and suppress expression of 17 genes including IL-6, PlGF, Daxx, and HSP27. These genes were found to be important in tumor progression through the mediation of inflammation, cell adhesion, proliferation, apoptosis and angiogenesis. In conclusion, our study provides supplementary information for the further studies on the pharmacological effects of sinomenine acting on synovial sarcoma.

AB - Sinomenine is an alkaloid with pharmacological effects of anti-inflammation, anti-angiogenesis, anti-arthritis and immunosuppression. This study aimed to investigate the effect of sinomenine on gene expression of human synovial sarcoma cells (Hs701.T) activated by IL-1β. The proliferative effect of sinomenine was examined in the presence or absence of IL-1β by the [3H]-thymidine incorporation and MTT assay, respectively. Using DNA microarray technology and RT-PCR, the activating action of IL-1β and modulatory effect of sinomenine on Hs701.T were simultaneously determined. Results showed that IL-1β could stimulate the proliferation and gene expression of Hs701.T cells. Sinomenine could significantly inhibit proliferation of IL-1β-activated Hs701.T cells and suppress expression of 17 genes including IL-6, PlGF, Daxx, and HSP27. These genes were found to be important in tumor progression through the mediation of inflammation, cell adhesion, proliferation, apoptosis and angiogenesis. In conclusion, our study provides supplementary information for the further studies on the pharmacological effects of sinomenine acting on synovial sarcoma.

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KW - Gene expression

KW - Hs701.T

KW - IL-1β

KW - Sinomenine

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JO - Life Sciences

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Effect of sinomenine on gene expression of the IL-1β-activated human synovial sarcoma

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