Economical Di-Rhamnolipids Biosynthesis by Non-Pathogenic Burkholderia thailandensis E264 Using Post-Consumption Food Waste in a Biorefinery Approach

Rajat Kumar, Davidraj Johnravindar, Jonathan W. C. Wong, Raffel Dharma Patria, Guneet Kaur*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

6 Citations (Scopus)

Abstract

Rhamnolipids (RLs) are one of the most promising eco-friendly green alternatives to commercially viable fossil fuel-based surfactants. However, the current bioprocess practices cannot meet the required affordability, quantity, and biocompatibility within an industrially relevant framework. To circumvent these issues, our study aims to develop a sustainable biorefinery approach using post-consumption food waste as a second-generation feedstock. In-depth substrate screening revealed that food waste hydrolysate (FWH) was rich in readily assimilable carbohydrates, volatile fatty acids, and amino acids. The fermentative valorization of FWH as a sole carbon and energy source with Burkholderis thailandensis E264 in a bioreactor showed active RLs biosynthesis of up to 0.6–0.8 g/L (34–40 mg/g FWH) in a short duration (72 h). In terms of the kinetic parameters, the FWH-RLs outperformed other supplemented pure/waste streams. Interestingly, the recovered RLs had a long chain length, with Rha-Rha-C12-C14 being the predominant isoform and exhibiting a strong emulsification ability (E24, 54.6%). To the best of our knowledge, this study is the first to prove bioreactor-level RLs production and their abundance in food waste. Moreover, the feasibility of this developed process could propel next-generation biosurfactants, lower waste burdens, and increase the industrial applicability of RLs, thereby significantly contributing to the development of a circular bioeconomy.
Original languageEnglish
Article number59
JournalSustainability
Volume15
Issue number1
Early online date21 Dec 2022
DOIs
Publication statusPublished - 1 Jan 2023

Scopus Subject Areas

  • Computer Science (miscellaneous)
  • Environmental Science (miscellaneous)
  • Geography, Planning and Development
  • Energy Engineering and Power Technology
  • Hardware and Architecture
  • Management, Monitoring, Policy and Law
  • Building and Construction
  • Computer Networks and Communications
  • Renewable Energy, Sustainability and the Environment

User-Defined Keywords

  • Burkholderia thailandensis
  • bioreactor
  • di-rhamnolipids
  • emulsification
  • second-generation feedstock
  • substrate screening

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