Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor

Víctor Fernández-Dueñas; Xavier Morató; Thomas Knöpfel; Francisco Ciruela

doi:10.1007/978-1-0716-1522-5_31

Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor

Víctor Fernández-Dueñas^*
, Xavier Morató
, Thomas Knöpfel
, Francisco Ciruela

^*Corresponding author for this work

Department of Physics

Research output: Chapter in book/report/conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Fluorescence-based biosensors for membrane voltage allow dynamic optical recording of neuronal activity. Interestingly, the development of genetically encoded voltage indicators constitutes a good alternative to classical voltage-sensitive dyes, since they allow overcoming some of the inherent problems (e.g., optical noise, etc.) associated to organic compounds. Here, we show the use of a genetically encoded voltage-sensitive fluorescent protein (VSFP), namely the VSFP2.32. This biosensor contains a mCerulean and Citrine tandem, which can engage in a constitutive fluorescent resonance energy transfer (FRET) process. We first expressed VSFP2.32 in hippocampal cultured neurons. And, subsequently, we monitored membrane voltage alterations in single neurons by recording (in a real-time mode) VSFP2.32 conformation-mediated FRET changes.

Original language	English
Title of host publication	Receptor and Ion Channel Detection in the Brain
Editors	Rafael Lujan, Francisco Ciruela
Publisher	Humana New York, NY
Chapter	31
Pages	523-530
Number of pages	8
Edition	2nd
ISBN (Electronic)	9781071615225
ISBN (Print)	9781071615218, 9781071615249
DOIs	https://doi.org/10.1007/978-1-0716-1522-5_31
Publication status	Published - 26 Jul 2021

Publication series

Name	Neuromethods
Publisher	Humana Press
Volume	169
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

FRET
Neuronal membrane potential
Voltage indicators

Access to Document

10.1007/978-1-0716-1522-5_31

Cite this

Fernández-Dueñas, V., Morató, X., Knöpfel, T., & Ciruela, F. (2021). Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor. In R. Lujan, & F. Ciruela (Eds.), Receptor and Ion Channel Detection in the Brain (2nd ed., pp. 523-530). (Neuromethods; Vol. 169). Humana New York, NY. https://doi.org/10.1007/978-1-0716-1522-5_31

Fernández-Dueñas, Víctor ; Morató, Xavier ; Knöpfel, Thomas et al. / Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor. Receptor and Ion Channel Detection in the Brain. editor / Rafael Lujan ; Francisco Ciruela. 2nd. ed. Humana New York, NY, 2021. pp. 523-530 (Neuromethods).

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abstract = "Fluorescence-based biosensors for membrane voltage allow dynamic optical recording of neuronal activity. Interestingly, the development of genetically encoded voltage indicators constitutes a good alternative to classical voltage-sensitive dyes, since they allow overcoming some of the inherent problems (e.g., optical noise, etc.) associated to organic compounds. Here, we show the use of a genetically encoded voltage-sensitive fluorescent protein (VSFP), namely the VSFP2.32. This biosensor contains a mCerulean and Citrine tandem, which can engage in a constitutive fluorescent resonance energy transfer (FRET) process. We first expressed VSFP2.32 in hippocampal cultured neurons. And, subsequently, we monitored membrane voltage alterations in single neurons by recording (in a real-time mode) VSFP2.32 conformation-mediated FRET changes.",

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Fernández-Dueñas, V, Morató, X, Knöpfel, T & Ciruela, F 2021, Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor. in R Lujan & F Ciruela (eds), Receptor and Ion Channel Detection in the Brain. 2nd edn, Neuromethods, vol. 169, Humana New York, NY, pp. 523-530. https://doi.org/10.1007/978-1-0716-1522-5_31

Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor. / Fernández-Dueñas, Víctor; Morató, Xavier; Knöpfel, Thomas et al.
Receptor and Ion Channel Detection in the Brain. ed. / Rafael Lujan; Francisco Ciruela. 2nd. ed. Humana New York, NY, 2021. p. 523-530 (Neuromethods; Vol. 169).

Research output: Chapter in book/report/conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor

AU - Fernández-Dueñas, Víctor

AU - Morató, Xavier

AU - Knöpfel, Thomas

AU - Ciruela, Francisco

PY - 2021/7/26

Y1 - 2021/7/26

N2 - Fluorescence-based biosensors for membrane voltage allow dynamic optical recording of neuronal activity. Interestingly, the development of genetically encoded voltage indicators constitutes a good alternative to classical voltage-sensitive dyes, since they allow overcoming some of the inherent problems (e.g., optical noise, etc.) associated to organic compounds. Here, we show the use of a genetically encoded voltage-sensitive fluorescent protein (VSFP), namely the VSFP2.32. This biosensor contains a mCerulean and Citrine tandem, which can engage in a constitutive fluorescent resonance energy transfer (FRET) process. We first expressed VSFP2.32 in hippocampal cultured neurons. And, subsequently, we monitored membrane voltage alterations in single neurons by recording (in a real-time mode) VSFP2.32 conformation-mediated FRET changes.

AB - Fluorescence-based biosensors for membrane voltage allow dynamic optical recording of neuronal activity. Interestingly, the development of genetically encoded voltage indicators constitutes a good alternative to classical voltage-sensitive dyes, since they allow overcoming some of the inherent problems (e.g., optical noise, etc.) associated to organic compounds. Here, we show the use of a genetically encoded voltage-sensitive fluorescent protein (VSFP), namely the VSFP2.32. This biosensor contains a mCerulean and Citrine tandem, which can engage in a constitutive fluorescent resonance energy transfer (FRET) process. We first expressed VSFP2.32 in hippocampal cultured neurons. And, subsequently, we monitored membrane voltage alterations in single neurons by recording (in a real-time mode) VSFP2.32 conformation-mediated FRET changes.

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KW - Neuronal membrane potential

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SN - 9781071615249

T3 - Neuromethods

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BT - Receptor and Ion Channel Detection in the Brain

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Fernández-Dueñas V, Morató X, Knöpfel T, Ciruela F. Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor. In Lujan R, Ciruela F, editors, Receptor and Ion Channel Detection in the Brain. 2nd ed. Humana New York, NY. 2021. p. 523-530. (Neuromethods). doi: 10.1007/978-1-0716-1522-5_31

Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor

Abstract

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