@inbook{cb62222b7f1f4a619a3cb5cbdfad29df,
title = "Dynamic recording of membrane potential from hippocampal neurons by using a FRET-based voltage biosensor",
abstract = "Fluorescence-based biosensors for membrane voltage (mV) allow dynamic optical recording of neuronal activity. Interestingly, the development of genetically encoded voltage indicators constitute a good alternative to classical voltage-sensitive dyes, thus allowing overcoming some of the inherent problems (e.g., optical noise, etc.) associated with these organic compounds. Here, we show the use of a genetically encoded voltage-sensitive fluorescent protein (VSFP), namely the VSFP2.32, which contains a mCerulean and Citrine tandem engaging in a constitutive fluorescent resonance energy transfer (FRET) process. By expressing VSFP2.32 in hippocampal cultured neurons, we were able to monitor mV alterations in single neurons by recording VSFP2.32 conformation-mediated FRET changes in a real-time mode.",
keywords = "FRET, Neuronal membrane potential, Voltage indicators",
author = "V{\'i}ctor Fern{\'a}ndez-Due{\~n}as and Xavier Morat{\'o} and Thomas Kn{\"o}pfel and Francisco Ciruela",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = feb,
day = "2",
doi = "10.1007/978-1-4939-3064-7_27",
language = "English",
isbn = "9781493930630",
series = "Neuromethods",
publisher = "Humana New York, NY",
pages = "447--454",
editor = "Rafael Luj{\'a}n and Francisco Ciruela",
booktitle = "Receptor and Ion Channel Detection in the Brain",
edition = "1st",
}
