@inbook{490a3d36fab64ef094f33fd964eb0b60,
title = "Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor",
abstract = "Fluorescence-based biosensors for membrane voltage allow dynamic optical recording of neuronal activity. Interestingly, the development of genetically encoded voltage indicators constitutes a good alternative to classical voltage-sensitive dyes, since they allow overcoming some of the inherent problems (e.g., optical noise, etc.) associated to organic compounds. Here, we show the use of a genetically encoded voltage-sensitive fluorescent protein (VSFP), namely the VSFP2.32. This biosensor contains a mCerulean and Citrine tandem, which can engage in a constitutive fluorescent resonance energy transfer (FRET) process. We first expressed VSFP2.32 in hippocampal cultured neurons. And, subsequently, we monitored membrane voltage alterations in single neurons by recording (in a real-time mode) VSFP2.32 conformation-mediated FRET changes.",
keywords = "FRET, Neuronal membrane potential, Voltage indicators",
author = "V{\'i}ctor Fern{\'a}ndez-Due{\~n}as and Xavier Morat{\'o} and Thomas Kn{\"o}pfel and Francisco Ciruela",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
month = jul,
day = "26",
doi = "10.1007/978-1-0716-1522-5_31",
language = "English",
isbn = "9781071615218",
series = "Neuromethods",
publisher = "Humana New York, NY",
pages = "523--530",
editor = "Rafael Lujan and Francisco Ciruela",
booktitle = "Receptor and Ion Channel Detection in the Brain",
edition = "2nd",
}
