Dynamic analysis of ABA accumulation in relation to the rate of ABA catabolism in maize tissues under water deficit

Huibo Ren, Zhihui Gao, Lin Chen, Kaifa Wei, Jing Liu, Yijuan Fan, William J. Davies, Wensuo Jia*, Jianhua ZHANG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

87 Citations (Scopus)

Abstract

The plant hormone abscisic acid (ABA) accumulates in plant tissues which experience water deficit (stress ABA). This study analysed its accumulation as a function of both synthesis and catabolism in maize tissues. By following the disappearance of the stress ABA when ABA synthesis was blocked by nordihydroguaiaretic acid (NDGA), the rate of the catabolism of stress ABA was determined. When compared with the catabolic rate of baseline (non-stress) ABA, stress ABA showed a catabolic rate >11 times higher. With such an elevated catabolic rate, it is proposed that the xanthophyll precursor pool may not be able to sustain the ABA accumulation, and such a proposition has been substantiated by further experiments where fluridone is used to limit the availability of upstream ABA precursors. When fluridone was used, stress ABA accumulation could only be sustained for a few hours, i.e. ∼5 h for leaf and 1 h for root tissues. In detached roots, stress ABA accumulation could not be sustained even if fluridone was not used, suggesting that stress ABA accumulation in root systems requires the continuous import of ABA precursors from the shoots. Such an assumption was substantiated by the observation that defoliation or shading significantly reduced ABA accumulation in intact roots. The present study suggests that ABA catabolism is rapid enough to play an important role in the regulation of ABA accumulation.

Original languageEnglish
Pages (from-to)211-219
Number of pages9
JournalJournal of Experimental Botany
Volume58
Issue number2
DOIs
Publication statusPublished - Jan 2007

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • ABA accumulation
  • ABA precursors
  • Catabolism
  • Maize
  • Water deficit

Fingerprint

Dive into the research topics of 'Dynamic analysis of ABA accumulation in relation to the rate of ABA catabolism in maize tissues under water deficit'. Together they form a unique fingerprint.

Cite this