Dual actions of brain-derived neurotrophic factor on GABAergic transmission in cerebellar Purkinje neurons

Ying Huang*, Ho Ko, Zelda H. Cheung, Kin Lam YUNG, Tai Yao, Jian Jun Wang, Alexei Morozov, Ya Ke, Nancy Y. Ip, Wing Ho Yung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The ability to regulate inhibitory synapses is a critical feature of the nervous system and a growing body of evidence indicates that brain-derived neurotrophic factor (BDNF) acutely modulates the efficacy of GABA synaptic transmission. Although the neuronal potassium-chloride cotransporter 2 (KCC2) has been implied in this BDNF-induced ionic plasticity, the reports about actions of BDNF on GABA signaling remain conflicting. Here we show dual effects of BDNF on GABAergic synaptic transmission in Purkinje neurons in rat cerebellar slices. BDNF decreased the amplitude of evoked outward IPSCs postsynaptically. It induced a depolarizing shift in the reversal potential (EIPSC), which reduced the driving force for outward IPSCs. However, in the absence of KCC2 activity, BDNF directly potentiated rather than inhibited GABAA receptor, which was reflected by an increase in the amplitude of outward IPSCs. This action of BDNF coincided with its effect in increasing the amplitude of inward IPSCs. Furthermore, an interaction between GABAA receptor and KCC2 was revealed by co-immunoprecipitation. The effects of BDNF on both GABAA receptor and KCC2 were dependent on TrkB and also activation of cyclin-dependent kinase 5 (Cdk5). However, only the effect of BDNF on KCC2 activity was dependent on a rise of intracellular calcium. Taken together, these data highlight distinct actions of BDNF on KCC2 and GABAA receptor in the regulation of GABAergic synaptic transmission.

Original languageEnglish
Pages (from-to)791-798
Number of pages8
JournalExperimental Neurology
Volume233
Issue number2
DOIs
Publication statusPublished - Feb 2012

Scopus Subject Areas

  • Neurology
  • Developmental Neuroscience

User-Defined Keywords

  • BDNF
  • Cdk5
  • KCC2
  • Purkinje neurons

Fingerprint

Dive into the research topics of 'Dual actions of brain-derived neurotrophic factor on GABAergic transmission in cerebellar Purkinje neurons'. Together they form a unique fingerprint.

Cite this