DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells

Faith A. Bazley; Amir Pourmorteza; Siddharth Gupta; Nikta Pashai; Candace Kerr; Angelo H. All

doi:10.1109/EMBC.2012.6345876

DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells

Faith A. Bazley^*
, Amir Pourmorteza
, Siddharth Gupta
, Nikta Pashai
, Candace Kerr
, Angelo H. All

^*Corresponding author for this work

Department of Chemistry

Research output: Chapter in book/report/conference proceeding › Conference proceeding › peer-review

17 Citations (Scopus)

Abstract

We describe the feasibility of using diffusion tensor magnetic resonance imaging (DT-MRI) to study a contusive model of rat spinal cord injury following human stem cell transplantation at and around the site of injury. Rats receiving either a laminectomy or contusion injury were transplanted with oligodendrocyte precursor cells (OPCs). During the course of the study, bioluminescence imaging (BLI; up to 100 days) and somatosensory evoked potentials (SSEPs; up to 42 days) were used to evaluate cell survival and functional outcomes. Spinal cords were then analyzed ex vivo upon termination using diffusion tensor imaging (DTI). Improvements in fractional anisotropy (FA) at day 100 post-transplantation corresponded with cell survival and functional SSEP improvements. Thus, we illustrate the feasibility of DTI for evaluating axonal integrity in SCI after cell replacement therapies, and we provide examples utilizing OPC transplantations in a contusion rat model.

Original language	English
Title of host publication	2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012
Place of Publication	San Diego
Publisher	IEEE
Pages	82-85
Number of pages	4
ISBN (Electronic)	9781457717871
ISBN (Print)	9781424441198
DOIs	https://doi.org/10.1109/EMBC.2012.6345876
Publication status	Published - 28 Aug 2012
Event	34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society - San Diego, United States Duration: 28 Aug 2012 → 1 Sept 2012 https://ieeexplore.ieee.org/xpl/conhome/6320834/proceeding (Conference proceeding)

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)	1094-687X
ISSN (Electronic)	1558-4615

Conference

Conference	34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Abbreviated title	EMBS 2012
Country/Territory	United States
City	San Diego
Period	28/08/12 → 1/09/12
Internet address	https://ieeexplore.ieee.org/xpl/conhome/6320834/proceeding (Conference proceeding)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1109/EMBC.2012.6345876

Cite this

Bazley, F. A., Pourmorteza, A., Gupta, S., Pashai, N., Kerr, C., & All, A. H. (2012). DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells. In 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012 (pp. 82-85). (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). IEEE. https://doi.org/10.1109/EMBC.2012.6345876

Bazley, Faith A. ; Pourmorteza, Amir ; Gupta, Siddharth et al. / DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells. 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012. San Diego : IEEE, 2012. pp. 82-85 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

@inproceedings{ddddedda54584252b5ab68326bc29b4b,

title = "DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells",

abstract = "We describe the feasibility of using diffusion tensor magnetic resonance imaging (DT-MRI) to study a contusive model of rat spinal cord injury following human stem cell transplantation at and around the site of injury. Rats receiving either a laminectomy or contusion injury were transplanted with oligodendrocyte precursor cells (OPCs). During the course of the study, bioluminescence imaging (BLI; up to 100 days) and somatosensory evoked potentials (SSEPs; up to 42 days) were used to evaluate cell survival and functional outcomes. Spinal cords were then analyzed ex vivo upon termination using diffusion tensor imaging (DTI). Improvements in fractional anisotropy (FA) at day 100 post-transplantation corresponded with cell survival and functional SSEP improvements. Thus, we illustrate the feasibility of DTI for evaluating axonal integrity in SCI after cell replacement therapies, and we provide examples utilizing OPC transplantations in a contusion rat model.",

author = "Bazley, \{Faith A.\} and Amir Pourmorteza and Siddharth Gupta and Nikta Pashai and Candace Kerr and All, \{Angelo H.\}",

note = "This work was supported by the Maryland Stem Cell Research Fund under Grants 2007-MSCRFII-0159-00 and 2009-MSCRFII-0091-00. Publisher Copyright: {\textcopyright}2012 IEEE; 34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2012 ; Conference date: 28-08-2012 Through 01-09-2012",

year = "2012",

month = aug,

day = "28",

doi = "10.1109/EMBC.2012.6345876",

language = "English",

isbn = "9781424441198",

series = "Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS",

publisher = "IEEE",

pages = "82--85",

booktitle = "2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012",

address = "United States",

url = "https://ieeexplore.ieee.org/xpl/conhome/6320834/proceeding",

}

Bazley, FA, Pourmorteza, A, Gupta, S, Pashai, N, Kerr, C & All, AH 2012, DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells. in 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, IEEE, San Diego, pp. 82-85, 34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, San Diego, California, United States, 28/08/12. https://doi.org/10.1109/EMBC.2012.6345876

DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells. / Bazley, Faith A.; Pourmorteza, Amir; Gupta, Siddharth et al.
2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012. San Diego: IEEE, 2012. p. 82-85 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in book/report/conference proceeding › Conference proceeding › peer-review

TY - GEN

T1 - DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells

AU - Bazley, Faith A.

AU - Pourmorteza, Amir

AU - Gupta, Siddharth

AU - Pashai, Nikta

AU - Kerr, Candace

AU - All, Angelo H.

PY - 2012/8/28

Y1 - 2012/8/28

N2 - We describe the feasibility of using diffusion tensor magnetic resonance imaging (DT-MRI) to study a contusive model of rat spinal cord injury following human stem cell transplantation at and around the site of injury. Rats receiving either a laminectomy or contusion injury were transplanted with oligodendrocyte precursor cells (OPCs). During the course of the study, bioluminescence imaging (BLI; up to 100 days) and somatosensory evoked potentials (SSEPs; up to 42 days) were used to evaluate cell survival and functional outcomes. Spinal cords were then analyzed ex vivo upon termination using diffusion tensor imaging (DTI). Improvements in fractional anisotropy (FA) at day 100 post-transplantation corresponded with cell survival and functional SSEP improvements. Thus, we illustrate the feasibility of DTI for evaluating axonal integrity in SCI after cell replacement therapies, and we provide examples utilizing OPC transplantations in a contusion rat model.

AB - We describe the feasibility of using diffusion tensor magnetic resonance imaging (DT-MRI) to study a contusive model of rat spinal cord injury following human stem cell transplantation at and around the site of injury. Rats receiving either a laminectomy or contusion injury were transplanted with oligodendrocyte precursor cells (OPCs). During the course of the study, bioluminescence imaging (BLI; up to 100 days) and somatosensory evoked potentials (SSEPs; up to 42 days) were used to evaluate cell survival and functional outcomes. Spinal cords were then analyzed ex vivo upon termination using diffusion tensor imaging (DTI). Improvements in fractional anisotropy (FA) at day 100 post-transplantation corresponded with cell survival and functional SSEP improvements. Thus, we illustrate the feasibility of DTI for evaluating axonal integrity in SCI after cell replacement therapies, and we provide examples utilizing OPC transplantations in a contusion rat model.

UR - https://www.scopus.com/pages/publications/84881067838

UR - https://ieeexplore.ieee.org/document/6345876

U2 - 10.1109/EMBC.2012.6345876

DO - 10.1109/EMBC.2012.6345876

M3 - Conference proceeding

C2 - 23365837

AN - SCOPUS:84881067838

SN - 9781424441198

T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS

SP - 82

EP - 85

BT - 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012

PB - IEEE

CY - San Diego

T2 - 34th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

Y2 - 28 August 2012 through 1 September 2012

ER -

Bazley FA, Pourmorteza A, Gupta S, Pashai N, Kerr C, All AH. DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells. In 2012 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2012. San Diego: IEEE. 2012. p. 82-85. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). doi: 10.1109/EMBC.2012.6345876

DTI for assessing axonal integrity after contusive spinal cord injury and transplantation of oligodendrocyte progenitor cells

Abstract

Publication series

Conference

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this