TY - JOUR
T1 - Drug screening strategies using metal-based luminescent probes
AU - Li, Guodong
AU - Wu, Chun
AU - Ma, Edmond Dik Lung
AU - Leung, Chung Hang
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/6
Y1 - 2021/6
N2 - Drug screening assays employing organic fluorophores are widely used for high-throughput screening of potential drugs. However, organic dyes generally possess short lifetimes and narrow Stokes shifts, and are susceptible to background signal interference. In recent years, metal-based luminescent probes, such as transition metal complexes (e.g. Ir(III), Ru(II)), lanthanide complexes (e.g. Eu(III) and Tb(III)), and metal-based nanomaterials or quantum dots, have found increasing use for biosensing and bioimaging owing to their distinct spectral and structural characteristics. In this review, we first introduce the photophysical properties of metal-based luminescent probes, and then describe representative examples of metal-based luminescent probes and their applications for drug screening, including competitive, oligonucleotide-based, reaction-based, and assembly-based drug screening strategies. We also present the combination of these metal-based probes with luminescence microscopy (e.g. TRES) or imaging techniques (e.g. PLIM), which could open new doors for translational biomedical and/or (pre)clinical studies of biomolecular regulators in more challenging biological systems.
AB - Drug screening assays employing organic fluorophores are widely used for high-throughput screening of potential drugs. However, organic dyes generally possess short lifetimes and narrow Stokes shifts, and are susceptible to background signal interference. In recent years, metal-based luminescent probes, such as transition metal complexes (e.g. Ir(III), Ru(II)), lanthanide complexes (e.g. Eu(III) and Tb(III)), and metal-based nanomaterials or quantum dots, have found increasing use for biosensing and bioimaging owing to their distinct spectral and structural characteristics. In this review, we first introduce the photophysical properties of metal-based luminescent probes, and then describe representative examples of metal-based luminescent probes and their applications for drug screening, including competitive, oligonucleotide-based, reaction-based, and assembly-based drug screening strategies. We also present the combination of these metal-based probes with luminescence microscopy (e.g. TRES) or imaging techniques (e.g. PLIM), which could open new doors for translational biomedical and/or (pre)clinical studies of biomolecular regulators in more challenging biological systems.
KW - Drug screening
KW - Luminescence
KW - Metal-based probes
UR - http://www.scopus.com/inward/record.url?scp=85103573331&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2021.116270
DO - 10.1016/j.trac.2021.116270
M3 - Review article
AN - SCOPUS:85103573331
SN - 0165-9936
VL - 139
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
M1 - 116270
ER -