Abstract
Aim: To explore the role of miR-181a-5p in the progression of acute kidney injury (AKI) to renal interstitial fibrosis (RIF) from the perspective of DNA methylation. Materials & methods: The role of miR-181a-5p was confirmed by collecting clinical samples, injecting miR-181a-5p agomir into tail vein, and transfecting miR-181a-5p mimic in vitro. The mechanism of miR-181a-5p’s influence on AKI induced RIF was investigated by methylation-specific PCR, bioinformatic analysis, transcriptome sequencing and so on. Results: MiR-181a-5p plays an important role in AKI induced RIF. DNMT3b-mediated miR-181a-5p promoter hypermethylation is the main reason for the downregulation of miR-181a-5p. HDAC9 and SNAI2 are direct targets of miR-181a-5p. Conclusion: Hypermethylation of miR-181a-5p promoter mediated by DNMT3b promotes AKI induced RIF by targeting HDAC9 and SNAI2.
Original language | English |
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Pages (from-to) | 945-960 |
Number of pages | 16 |
Journal | Epigenomics |
Volume | 16 |
Issue number | 13 |
Early online date | 18 Jul 2024 |
DOIs | |
Publication status | Published - Sept 2024 |
Scopus Subject Areas
- Genetics
- Cancer Research
User-Defined Keywords
- acute kidney injury
- DNMT3b
- HDAC9
- miR-181a-5p
- renal interstitial fibrosis
- SNAI2