Distribution of Sodium and Potassium Within Individual Human Erythrocytes by Pulsed-Laser Vaporization in a Sheath Flow

Nai Ho CHEUNG*, Edward S. Yeung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Simultaneous determination of the amounts of Na and K inside single human erythrocytes was accomplished by laser vaporization and monitoring the atomic emission produced. By using a modified sheath-flow arrangement, detection of 8 fg (0.35 fmol) of Na is possible with one laser pulse. By using Poisson statistics, one can obtain single-cell information even when multiple cells are vaporized per laser pulse. The intracellular contents for a given individual were found to vary significantly. The ±55% and ±155% variations for Na and K, respectively, cannot be explained by changes in cell volume. There is only a weak correlation between the Na and K contents in single cells. The results reflect the age distribution of erythrocytes in the sample. Presumably, the enzymes regulating ion transport lose their activities in the older cells.

Original languageEnglish
Pages (from-to)929-936
Number of pages8
JournalAnalytical Chemistry
Volume66
Issue number7
DOIs
Publication statusPublished - 20 Apr 1994

Scopus Subject Areas

  • Analytical Chemistry

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