Disruption of the arabidopsis defense regulator genes SAG101, EDS1, and PAD4 confers enhanced freezing tolerance

Qin Fang Chen, Le Xu, Wei Juan Tan, Liang Chen, Hua Qi, Li Juan Xie, Mo Xian Chen, Bin Yi Liu, Lu Jun Yu, Nan Yao, Jianhua ZHANG, Wensheng Shu, Shi Xiao*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

In Arabidopsis, three lipase-like regulators, SAG101, EDS1, and PAD4, act downstream of resistance protein-associated defense signaling. Although the roles of SAG101, EDS1, and PAD4 in biotic stress have been extensively studied, little is known about their functions in plant responses to abiotic stresses. Here, we show that SAG101, EDS1, and PAD4 are involved in the regulation of freezing tolerance in Arabidopsis. With or without cold acclimation, the sag101, eds1, and pad4 single mutants, as well as their double mutants, exhibited similarly enhanced tolerance to freezing temperatures. Upon cold exposure, the sag101, eds1, and pad4 mutants showed increased transcript levels of C-REPEAT/DRE BINDING FACTORs and their regulons compared with the wild type. Moreover, freezing-induced cell death and accumulation of hydrogen peroxide were ameliorated in sag101, eds1, and pad4 mutants. The sag101, eds1, and pad4 mutants had much lower salicylic acid (SA) and diacylglycerol (DAG) contents than the wild type, and exogenous application of SA and DAG compromised the freezing tolerance of the mutants. Furthermore, SA suppressed the cold-induced expression of DGATs and DGKs in the wild-type leaves. These findings indicate that SAG101, EDS1, and PAD4 are involved in the freezing response in Arabidopsis, at least in part, by modulating the homeostasis of SA and DAG.

Original languageEnglish
Pages (from-to)1536-1549
Number of pages14
JournalMolecular Plant
Volume8
Issue number10
DOIs
Publication statusPublished - 5 Oct 2015

Scopus Subject Areas

  • Molecular Biology
  • Plant Science

User-Defined Keywords

  • cold acclimation
  • diacylglycerol
  • freezing tolerance
  • reactive oxygen species
  • salicylic acid

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