Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation

Hongmei Cao; Rui Yu; Yongsoo Choi; Zhong Ze Ma; Hongjie Zhang; Wei Xiang; David Yue Wei Lee; Brian M. Berman; Kamal D. Moudgil; Harry H.S. Fong; Richard B. van Breemen

doi:10.1016/j.phrs.2010.02.007

Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation

Hongmei Cao, Rui Yu, Yongsoo Choi, Zhong Ze Ma, Hongjie Zhang, Wei Xiang, David Yue Wei Lee, Brian M. Berman, Kamal D. Moudgil, Harry H.S. Fong, Richard B. van Breemen^*

^*Corresponding author for this work

Chinese Medicine - Teaching and Research Division

Research output: Contribution to journal › Journal article › peer-review

101 Citations (Scopus)

Abstract

Eleven authenticated botanicals used in the traditional Chinese medicine Huo-Luo-Xiao-Ling Dan were screened for ligands to cyclooxygenase (COX) using pulsed ultrafiltration liquid chromatography-mass spectrometry, and a mass spectrometry-based enzyme assay was used to determine the concentration of each of 17 ligands that inhibited COX-1 or COX-2 by 50% (IC₅₀). Acetyl-11-keto-β-boswellic acid, β-boswellic acid, acetyl-α-boswellic acid, acetyl-β-boswellic acid, and betulinic acid were COX-1 selective inhibitors with IC₅₀ values of approximately 10μM. Senkyunolide O and cryptotanshinone were COX-2 selective inhibitors with IC₅₀ values of 5μM and 22μM, respectively. Roburic acid and phenethyl-trans-ferulate inhibited COX-1 and COX-2 equally. COX inhibition and the IC₅₀ values of most of these natural product ligands have not been reported previously.

Original language	English
Pages (from-to)	519-524
Number of pages	6
Journal	Pharmacological Research
Volume	61
Issue number	6
DOIs	https://doi.org/10.1016/j.phrs.2010.02.007
Publication status	Published - Jun 2010

Scopus Subject Areas

Pharmacology

User-Defined Keywords

Botanical dietary supplements
COX-2
Cryptotanshinone
Cyclooxygenase
Drug discovery
Senkyunolide O

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10.1016/j.phrs.2010.02.007

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@article{c2ca384290404a6f85838b0f93b81d7e,

title = "Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation",

abstract = "Eleven authenticated botanicals used in the traditional Chinese medicine Huo-Luo-Xiao-Ling Dan were screened for ligands to cyclooxygenase (COX) using pulsed ultrafiltration liquid chromatography-mass spectrometry, and a mass spectrometry-based enzyme assay was used to determine the concentration of each of 17 ligands that inhibited COX-1 or COX-2 by 50% (IC50). Acetyl-11-keto-β-boswellic acid, β-boswellic acid, acetyl-α-boswellic acid, acetyl-β-boswellic acid, and betulinic acid were COX-1 selective inhibitors with IC50 values of approximately 10μM. Senkyunolide O and cryptotanshinone were COX-2 selective inhibitors with IC50 values of 5μM and 22μM, respectively. Roburic acid and phenethyl-trans-ferulate inhibited COX-1 and COX-2 equally. COX inhibition and the IC50 values of most of these natural product ligands have not been reported previously.",

keywords = "Botanical dietary supplements, COX-2, Cryptotanshinone, Cyclooxygenase, Drug discovery, Senkyunolide O",

author = "Hongmei Cao and Rui Yu and Yongsoo Choi and Ma, {Zhong Ze} and Hongjie Zhang and Wei Xiang and Lee, {David Yue Wei} and Berman, {Brian M.} and Moudgil, {Kamal D.} and Fong, {Harry H.S.} and {van Breemen}, {Richard B.}",

note = "Funding Information: This research was supported by grants P01 CA48112 from the National Cancer Institute (NCI) and P01 AT002605 and P50 AT00155 from the National Center for Complementary and Alternative Medicine (NCCAM) , the Office of Dietary Supplements (ODS) and the Office for Research on Women's Health (ORWH) of the National Institutes of Health (NIH) . The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the NCI, NCCAM, ODS, ORWH, or the NIH.",

year = "2010",

month = jun,

doi = "10.1016/j.phrs.2010.02.007",

language = "English",

volume = "61",

pages = "519--524",

journal = "Pharmacological Research",

issn = "1043-6618",

publisher = "Elsevier",

number = "6",

}

TY - JOUR

T1 - Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation

AU - Cao, Hongmei

AU - Yu, Rui

AU - Choi, Yongsoo

AU - Ma, Zhong Ze

AU - Zhang, Hongjie

AU - Xiang, Wei

AU - Lee, David Yue Wei

AU - Berman, Brian M.

AU - Moudgil, Kamal D.

AU - Fong, Harry H.S.

AU - van Breemen, Richard B.

N1 - Funding Information: This research was supported by grants P01 CA48112 from the National Cancer Institute (NCI) and P01 AT002605 and P50 AT00155 from the National Center for Complementary and Alternative Medicine (NCCAM) , the Office of Dietary Supplements (ODS) and the Office for Research on Women's Health (ORWH) of the National Institutes of Health (NIH) . The contents of this paper are solely the responsibility of the authors and do not necessarily represent the official views of the NCI, NCCAM, ODS, ORWH, or the NIH.

PY - 2010/6

Y1 - 2010/6

N2 - Eleven authenticated botanicals used in the traditional Chinese medicine Huo-Luo-Xiao-Ling Dan were screened for ligands to cyclooxygenase (COX) using pulsed ultrafiltration liquid chromatography-mass spectrometry, and a mass spectrometry-based enzyme assay was used to determine the concentration of each of 17 ligands that inhibited COX-1 or COX-2 by 50% (IC50). Acetyl-11-keto-β-boswellic acid, β-boswellic acid, acetyl-α-boswellic acid, acetyl-β-boswellic acid, and betulinic acid were COX-1 selective inhibitors with IC50 values of approximately 10μM. Senkyunolide O and cryptotanshinone were COX-2 selective inhibitors with IC50 values of 5μM and 22μM, respectively. Roburic acid and phenethyl-trans-ferulate inhibited COX-1 and COX-2 equally. COX inhibition and the IC50 values of most of these natural product ligands have not been reported previously.

AB - Eleven authenticated botanicals used in the traditional Chinese medicine Huo-Luo-Xiao-Ling Dan were screened for ligands to cyclooxygenase (COX) using pulsed ultrafiltration liquid chromatography-mass spectrometry, and a mass spectrometry-based enzyme assay was used to determine the concentration of each of 17 ligands that inhibited COX-1 or COX-2 by 50% (IC50). Acetyl-11-keto-β-boswellic acid, β-boswellic acid, acetyl-α-boswellic acid, acetyl-β-boswellic acid, and betulinic acid were COX-1 selective inhibitors with IC50 values of approximately 10μM. Senkyunolide O and cryptotanshinone were COX-2 selective inhibitors with IC50 values of 5μM and 22μM, respectively. Roburic acid and phenethyl-trans-ferulate inhibited COX-1 and COX-2 equally. COX inhibition and the IC50 values of most of these natural product ligands have not been reported previously.

KW - Botanical dietary supplements

KW - COX-2

KW - Cryptotanshinone

KW - Cyclooxygenase

KW - Drug discovery

KW - Senkyunolide O

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U2 - 10.1016/j.phrs.2010.02.007

DO - 10.1016/j.phrs.2010.02.007

M3 - Journal article

C2 - 20188172

AN - SCOPUS:77952886752

SN - 1043-6618

VL - 61

SP - 519

EP - 524

JO - Pharmacological Research

JF - Pharmacological Research

IS - 6

ER -

Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation

Abstract

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