Direct immunomagnetic detection of low abundance cardiac biomarker by aptamer DNA nanocomplex

Ka Wang Wong; Di Xu; Dinggeng He; Man Shing Wong; Hung Wing Li

doi:10.1016/j.snb.2019.04.035

Direct immunomagnetic detection of low abundance cardiac biomarker by aptamer DNA nanocomplex

Ka Wang Wong, Di Xu, Dinggeng He, Man Shing Wong^*, Hung Wing Li^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

11 Citations (Scopus)

34 Downloads (Pure)

Abstract

An early and rapid diagnosis of acute myocardial infraction (MI) by serum biomarker quantification is vital for boosting up treatment efficacy and survival rate; however, it still remains challenging due to ultralow biomarkers content and the emergent nature of the disease. In view of these challenges, a single-step target capturing method for cardiac Troponin I (cTnI), the major cardiac biomarker of MI, was developed. The detection of target cTnI was performed by coupling the capture antibody modified magnetic silica nanoparticle (mSiO₂) and an aptamer-based DNA nanostructure produced by hybridization chain reaction (aptHCR). cTnI in sample was first directly captured by mSiO₂ and aptHCR in 30 min. The as formed nanocomplex was then labelled by a customized DNA turn on fluorescent dye SPM. By reading the strengthened fluorescent signal using total internal reflection fluorescence microscope (TIRFM), the detection platform has accomplished an impressive LOD of 8.5 fM with high specificity towards cTnI. The advantages of the short immunoreaction time (30 min) and the pre-treatment free property of the method have addressed the urgent diagnostic requirements of MI. The detection platform offers a potential solution to tackle insufficient sensitivity and tedious turnaround time of clinically approved assays for cTnI detection.

Original language	English
Pages (from-to)	200-206
Number of pages	7
Journal	Sensors and Actuators B: Chemical
Volume	291
DOIs	https://doi.org/10.1016/j.snb.2019.04.035
Publication status	Published - 15 Jul 2019

Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

User-Defined Keywords

Aptamer
Cardiac troponin I (cTnI)
Hybridization Chain Reaction (HCR)
One step target capture
Turn on fluorescent dye

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10.1016/j.snb.2019.04.035

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Cite this

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title = "Direct immunomagnetic detection of low abundance cardiac biomarker by aptamer DNA nanocomplex",

abstract = "An early and rapid diagnosis of acute myocardial infraction (MI) by serum biomarker quantification is vital for boosting up treatment efficacy and survival rate; however, it still remains challenging due to ultralow biomarkers content and the emergent nature of the disease. In view of these challenges, a single-step target capturing method for cardiac Troponin I (cTnI), the major cardiac biomarker of MI, was developed. The detection of target cTnI was performed by coupling the capture antibody modified magnetic silica nanoparticle (mSiO2) and an aptamer-based DNA nanostructure produced by hybridization chain reaction (aptHCR). cTnI in sample was first directly captured by mSiO2 and aptHCR in 30 min. The as formed nanocomplex was then labelled by a customized DNA turn on fluorescent dye SPM. By reading the strengthened fluorescent signal using total internal reflection fluorescence microscope (TIRFM), the detection platform has accomplished an impressive LOD of 8.5 fM with high specificity towards cTnI. The advantages of the short immunoreaction time (30 min) and the pre-treatment free property of the method have addressed the urgent diagnostic requirements of MI. The detection platform offers a potential solution to tackle insufficient sensitivity and tedious turnaround time of clinically approved assays for cTnI detection.",

keywords = "Aptamer, Cardiac troponin I (cTnI), Hybridization Chain Reaction (HCR), One step target capture, Turn on fluorescent dye",

author = "Wong, {Ka Wang} and Di Xu and Dinggeng He and Wong, {Man Shing} and Li, {Hung Wing}",

note = "Funding Information: This work was supported by the Collaborative Research Fund of Hong Kong Research Grant Council [ C2012-15G ], the General Research Fund [ 12301317 and 12308416 ], and the National Natural Science Foundation of China [No. 21675135]. Publisher copyright: {\textcopyright} 2019 Elsevier B.V. All rights reserved.",

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AU - Wong, Ka Wang

AU - Xu, Di

AU - He, Dinggeng

AU - Wong, Man Shing

AU - Li, Hung Wing

N1 - Funding Information: This work was supported by the Collaborative Research Fund of Hong Kong Research Grant Council [ C2012-15G ], the General Research Fund [ 12301317 and 12308416 ], and the National Natural Science Foundation of China [No. 21675135]. Publisher copyright: © 2019 Elsevier B.V. All rights reserved.

PY - 2019/7/15

Y1 - 2019/7/15

N2 - An early and rapid diagnosis of acute myocardial infraction (MI) by serum biomarker quantification is vital for boosting up treatment efficacy and survival rate; however, it still remains challenging due to ultralow biomarkers content and the emergent nature of the disease. In view of these challenges, a single-step target capturing method for cardiac Troponin I (cTnI), the major cardiac biomarker of MI, was developed. The detection of target cTnI was performed by coupling the capture antibody modified magnetic silica nanoparticle (mSiO2) and an aptamer-based DNA nanostructure produced by hybridization chain reaction (aptHCR). cTnI in sample was first directly captured by mSiO2 and aptHCR in 30 min. The as formed nanocomplex was then labelled by a customized DNA turn on fluorescent dye SPM. By reading the strengthened fluorescent signal using total internal reflection fluorescence microscope (TIRFM), the detection platform has accomplished an impressive LOD of 8.5 fM with high specificity towards cTnI. The advantages of the short immunoreaction time (30 min) and the pre-treatment free property of the method have addressed the urgent diagnostic requirements of MI. The detection platform offers a potential solution to tackle insufficient sensitivity and tedious turnaround time of clinically approved assays for cTnI detection.

AB - An early and rapid diagnosis of acute myocardial infraction (MI) by serum biomarker quantification is vital for boosting up treatment efficacy and survival rate; however, it still remains challenging due to ultralow biomarkers content and the emergent nature of the disease. In view of these challenges, a single-step target capturing method for cardiac Troponin I (cTnI), the major cardiac biomarker of MI, was developed. The detection of target cTnI was performed by coupling the capture antibody modified magnetic silica nanoparticle (mSiO2) and an aptamer-based DNA nanostructure produced by hybridization chain reaction (aptHCR). cTnI in sample was first directly captured by mSiO2 and aptHCR in 30 min. The as formed nanocomplex was then labelled by a customized DNA turn on fluorescent dye SPM. By reading the strengthened fluorescent signal using total internal reflection fluorescence microscope (TIRFM), the detection platform has accomplished an impressive LOD of 8.5 fM with high specificity towards cTnI. The advantages of the short immunoreaction time (30 min) and the pre-treatment free property of the method have addressed the urgent diagnostic requirements of MI. The detection platform offers a potential solution to tackle insufficient sensitivity and tedious turnaround time of clinically approved assays for cTnI detection.

KW - Aptamer

KW - Cardiac troponin I (cTnI)

KW - Hybridization Chain Reaction (HCR)

KW - One step target capture

KW - Turn on fluorescent dye

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JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

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Direct immunomagnetic detection of low abundance cardiac biomarker by aptamer DNA nanocomplex

Abstract

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