TY - JOUR
T1 - Direct detection of prostate specific antigen by darkfield microscopy using single immunotargeting silver nanoparticle
AU - Poon, Chung Yan
AU - Chan, Ho Man
AU - LI, Hung Wing
N1 - Funding Information:
This work is supported by the General Research Fund from the Research Grant Council of the Hong Kong Special Administrative Region ( HKBU 201309, HKBU 201612 ) and the Young Scientists Fund from the National Science Foundation of China ( 21205006 ).The FEI-Technai G2 TEM used in this work was supported by the Institute of Advanced Materials of the Hong Kong Baptist University with funding from the Special Equipment Grant from the University Grants Committee of the Hong Kong Special Administrative Region, China ( SEG_HKBU06 ). We thank Mr. Benson Leung for the TEM measurement.
PY - 2014
Y1 - 2014
N2 - This work demonstrated a sensitive but direct method in detecting cancer biomarkers using dark field microscopy (DFM). Prostate-specific antigen (PSA), a well-known biomarker for prostate cancer, was chosen as the analyte to prove the concept. The immunoassay-based detection was performed in glass flow cell. Antibody-conjugated silver nanoparticles (AgNP-Ab) were served as the probe to capture PSA and then formed AgNP-Ab-PSA complexes on the surface of flow cell. The number of complexes is corresponding to the amount of PSA, which can be quantified by nanoparticle counting with DFM coupled with charge-coupled device (CCD) camera. The detection limit at 9 pM of this assay is well below the PSA threshold of prostate cancer patient, suggested the feasibility of our assay in diagnosis application.
AB - This work demonstrated a sensitive but direct method in detecting cancer biomarkers using dark field microscopy (DFM). Prostate-specific antigen (PSA), a well-known biomarker for prostate cancer, was chosen as the analyte to prove the concept. The immunoassay-based detection was performed in glass flow cell. Antibody-conjugated silver nanoparticles (AgNP-Ab) were served as the probe to capture PSA and then formed AgNP-Ab-PSA complexes on the surface of flow cell. The number of complexes is corresponding to the amount of PSA, which can be quantified by nanoparticle counting with DFM coupled with charge-coupled device (CCD) camera. The detection limit at 9 pM of this assay is well below the PSA threshold of prostate cancer patient, suggested the feasibility of our assay in diagnosis application.
KW - Darkfield microscopy
KW - Immunosensor
KW - Prostate specific antigen
KW - Silver nanoparticle
KW - Single particle counting
UR - http://www.scopus.com/inward/record.url?scp=84885574794&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2013.09.057
DO - 10.1016/j.snb.2013.09.057
M3 - Article
AN - SCOPUS:84885574794
SN - 0925-4005
VL - 190
SP - 737
EP - 744
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -
