Direct detection of prostate specific antigen by darkfield microscopy using single immunotargeting silver nanoparticle

Chung Yan Poon; Ho Man Chan; Hung Wing Li

doi:10.1016/j.snb.2013.09.057

Direct detection of prostate specific antigen by darkfield microscopy using single immunotargeting silver nanoparticle

Chung Yan Poon, Ho Man Chan, Hung Wing Li^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

25 Citations (Scopus)

Abstract

This work demonstrated a sensitive but direct method in detecting cancer biomarkers using dark field microscopy (DFM). Prostate-specific antigen (PSA), a well-known biomarker for prostate cancer, was chosen as the analyte to prove the concept. The immunoassay-based detection was performed in glass flow cell. Antibody-conjugated silver nanoparticles (AgNP-Ab) were served as the probe to capture PSA and then formed AgNP-Ab-PSA complexes on the surface of flow cell. The number of complexes is corresponding to the amount of PSA, which can be quantified by nanoparticle counting with DFM coupled with charge-coupled device (CCD) camera. The detection limit at 9 pM of this assay is well below the PSA threshold of prostate cancer patient, suggested the feasibility of our assay in diagnosis application.

Original language	English
Pages (from-to)	737-744
Number of pages	8
Journal	Sensors and Actuators B: Chemical
Volume	190
DOIs	https://doi.org/10.1016/j.snb.2013.09.057
Publication status	Published - Jan 2014

Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

User-Defined Keywords

Darkfield microscopy
Immunosensor
Prostate specific antigen
Silver nanoparticle
Single particle counting

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2013.09.057

Cite this

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title = "Direct detection of prostate specific antigen by darkfield microscopy using single immunotargeting silver nanoparticle",

abstract = "This work demonstrated a sensitive but direct method in detecting cancer biomarkers using dark field microscopy (DFM). Prostate-specific antigen (PSA), a well-known biomarker for prostate cancer, was chosen as the analyte to prove the concept. The immunoassay-based detection was performed in glass flow cell. Antibody-conjugated silver nanoparticles (AgNP-Ab) were served as the probe to capture PSA and then formed AgNP-Ab-PSA complexes on the surface of flow cell. The number of complexes is corresponding to the amount of PSA, which can be quantified by nanoparticle counting with DFM coupled with charge-coupled device (CCD) camera. The detection limit at 9 pM of this assay is well below the PSA threshold of prostate cancer patient, suggested the feasibility of our assay in diagnosis application.",

keywords = "Darkfield microscopy, Immunosensor, Prostate specific antigen, Silver nanoparticle, Single particle counting",

author = "Poon, {Chung Yan} and Chan, {Ho Man} and Li, {Hung Wing}",

note = "Funding Information: This work is supported by the General Research Fund from the Research Grant Council of the Hong Kong Special Administrative Region ( HKBU 201309, HKBU 201612 ) and the Young Scientists Fund from the National Science Foundation of China ( 21205006 ).The FEI-Technai G2 TEM used in this work was supported by the Institute of Advanced Materials of the Hong Kong Baptist University with funding from the Special Equipment Grant from the University Grants Committee of the Hong Kong Special Administrative Region, China ( SEG_HKBU06 ). We thank Mr. Benson Leung for the TEM measurement. ",

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doi = "10.1016/j.snb.2013.09.057",

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AU - Poon, Chung Yan

AU - Chan, Ho Man

AU - Li, Hung Wing

N1 - Funding Information: This work is supported by the General Research Fund from the Research Grant Council of the Hong Kong Special Administrative Region ( HKBU 201309, HKBU 201612 ) and the Young Scientists Fund from the National Science Foundation of China ( 21205006 ).The FEI-Technai G2 TEM used in this work was supported by the Institute of Advanced Materials of the Hong Kong Baptist University with funding from the Special Equipment Grant from the University Grants Committee of the Hong Kong Special Administrative Region, China ( SEG_HKBU06 ). We thank Mr. Benson Leung for the TEM measurement.

PY - 2014/1

Y1 - 2014/1

N2 - This work demonstrated a sensitive but direct method in detecting cancer biomarkers using dark field microscopy (DFM). Prostate-specific antigen (PSA), a well-known biomarker for prostate cancer, was chosen as the analyte to prove the concept. The immunoassay-based detection was performed in glass flow cell. Antibody-conjugated silver nanoparticles (AgNP-Ab) were served as the probe to capture PSA and then formed AgNP-Ab-PSA complexes on the surface of flow cell. The number of complexes is corresponding to the amount of PSA, which can be quantified by nanoparticle counting with DFM coupled with charge-coupled device (CCD) camera. The detection limit at 9 pM of this assay is well below the PSA threshold of prostate cancer patient, suggested the feasibility of our assay in diagnosis application.

AB - This work demonstrated a sensitive but direct method in detecting cancer biomarkers using dark field microscopy (DFM). Prostate-specific antigen (PSA), a well-known biomarker for prostate cancer, was chosen as the analyte to prove the concept. The immunoassay-based detection was performed in glass flow cell. Antibody-conjugated silver nanoparticles (AgNP-Ab) were served as the probe to capture PSA and then formed AgNP-Ab-PSA complexes on the surface of flow cell. The number of complexes is corresponding to the amount of PSA, which can be quantified by nanoparticle counting with DFM coupled with charge-coupled device (CCD) camera. The detection limit at 9 pM of this assay is well below the PSA threshold of prostate cancer patient, suggested the feasibility of our assay in diagnosis application.

KW - Darkfield microscopy

KW - Immunosensor

KW - Prostate specific antigen

KW - Silver nanoparticle

KW - Single particle counting

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DO - 10.1016/j.snb.2013.09.057

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SN - 0925-4005

VL - 190

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JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

ER -

Direct detection of prostate specific antigen by darkfield microscopy using single immunotargeting silver nanoparticle

Abstract

Scopus Subject Areas

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UN SDGs

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