Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes

Kathryn L. Gempf; Stephen J. Butler; Alexander M. Funk; David Parker

doi:10.1039/c3cc45875j

Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes

Kathryn L. Gempf, Stephen J. Butler^*, Alexander M. Funk, David Parker^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

34 Citations (Scopus)

Abstract

A cysteine-selective tagging method in water is reported, based on rapid displacement of a pyridyl nitro-substituent in simple pyridines and lanthanide complexes. The conjugation reaction creates a short link between the tag and peptide, holding the peptide closer to the Ln³⁺ ion and with reduced flexibility compared to existing methods.

Original language	English
Pages (from-to)	9104-9106
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	80
Early online date	21 Aug 2013
DOIs	https://doi.org/10.1039/c3cc45875j
Publication status	Published - 14 Oct 2013

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title = "Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes",

abstract = "A cysteine-selective tagging method in water is reported, based on rapid displacement of a pyridyl nitro-substituent in simple pyridines and lanthanide complexes. The conjugation reaction creates a short link between the tag and peptide, holding the peptide closer to the Ln3+ ion and with reduced flexibility compared to existing methods.",

author = "Gempf, {Kathryn L.} and Butler, {Stephen J.} and Funk, {Alexander M.} and David Parker",

note = "We thank Dr Jackie Mosely for analysis of the MALDI MS data and the ERC for support (FCC 266804). Publisher Copyright: {\textcopyright}The Royal Society of Chemistry 2013",

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language = "English",

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TY - JOUR

T1 - Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes

AU - Gempf, Kathryn L.

AU - Butler, Stephen J.

AU - Funk, Alexander M.

AU - Parker, David

N1 - We thank Dr Jackie Mosely for analysis of the MALDI MS data and the ERC for support (FCC 266804). Publisher Copyright: ©The Royal Society of Chemistry 2013

PY - 2013/10/14

Y1 - 2013/10/14

N2 - A cysteine-selective tagging method in water is reported, based on rapid displacement of a pyridyl nitro-substituent in simple pyridines and lanthanide complexes. The conjugation reaction creates a short link between the tag and peptide, holding the peptide closer to the Ln3+ ion and with reduced flexibility compared to existing methods.

AB - A cysteine-selective tagging method in water is reported, based on rapid displacement of a pyridyl nitro-substituent in simple pyridines and lanthanide complexes. The conjugation reaction creates a short link between the tag and peptide, holding the peptide closer to the Ln3+ ion and with reduced flexibility compared to existing methods.

UR - http://www.scopus.com/inward/record.url?scp=84884180788&partnerID=8YFLogxK

UR - https://pubs.rsc.org/en/content/articlelanding/2013/cc/c3cc45875j

U2 - 10.1039/c3cc45875j

DO - 10.1039/c3cc45875j

M3 - Journal article

C2 - 23989726

AN - SCOPUS:84884180788

SN - 1359-7345

VL - 49

SP - 9104

EP - 9106

JO - Chemical Communications

JF - Chemical Communications

IS - 80

ER -

Direct and selective tagging of cysteine residues in peptides and proteins with 4-nitropyridyl lanthanide complexes

Abstract

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