Direct and selective nanosensor for amyloid-β oligomers in serum for Alzheimer's disease diagnosis

Qingting Song; Hailong Zhang; Yue Lan; Jia Kong; Man Shing Wong; Hung Wing Li

doi:10.1039/d5ra01020a

Direct and selective nanosensor for amyloid-β oligomers in serum for Alzheimer's disease diagnosis

Qingting Song, Hailong Zhang, Yue Lan, Jia Kong, Man Shing Wong^*, Hung Wing Li^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

1 Citation (Scopus)

Abstract

The rising prevalence of Alzheimer's disease (AD) highlights an urgent need for ultra-sensitive diagnostic tools that facilitate early detection and intervention. Soluble Aβ oligomers (AβOs) have emerged as a key focus due to their specificity for AD pathology. This study introduces a magnetic nanoplatform-based ultrasensitive diagnostic assay for AβO detection, utilizing a novel custom-designed AβO-selective fluorophore, named O-SLM. This innovative approach achieves a sensitivity nearly tenfold greater than that of a comparative ELISA kit, with a detection limit as low as 25 fM. The assay requires minimal sample volumes and streamlines the protocol by omitting detection antibodies. It effectively distinguishes AD patients from healthy individuals through serum AβO quantification, demonstrating the versatility of our ultra-sensitive assay across various biomarkers.

Original language	English
Pages (from-to)	16175-16182
Number of pages	8
Journal	RSC Advances
Volume	15
Issue number	20
DOIs	https://doi.org/10.1039/d5ra01020a
Publication status	Published - 15 May 2025

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title = "Direct and selective nanosensor for amyloid-β oligomers in serum for Alzheimer's disease diagnosis",

abstract = "The rising prevalence of Alzheimer's disease (AD) highlights an urgent need for ultra-sensitive diagnostic tools that facilitate early detection and intervention. Soluble Aβ oligomers (AβOs) have emerged as a key focus due to their specificity for AD pathology. This study introduces a magnetic nanoplatform-based ultrasensitive diagnostic assay for AβO detection, utilizing a novel custom-designed AβO-selective fluorophore, named O-SLM. This innovative approach achieves a sensitivity nearly tenfold greater than that of a comparative ELISA kit, with a detection limit as low as 25 fM. The assay requires minimal sample volumes and streamlines the protocol by omitting detection antibodies. It effectively distinguishes AD patients from healthy individuals through serum AβO quantification, demonstrating the versatility of our ultra-sensitive assay across various biomarkers.",

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T1 - Direct and selective nanosensor for amyloid-β oligomers in serum for Alzheimer's disease diagnosis

AU - Song, Qingting

AU - Zhang, Hailong

AU - Lan, Yue

AU - Kong, Jia

AU - Wong, Man Shing

AU - Li, Hung Wing

PY - 2025/5/15

Y1 - 2025/5/15

N2 - The rising prevalence of Alzheimer's disease (AD) highlights an urgent need for ultra-sensitive diagnostic tools that facilitate early detection and intervention. Soluble Aβ oligomers (AβOs) have emerged as a key focus due to their specificity for AD pathology. This study introduces a magnetic nanoplatform-based ultrasensitive diagnostic assay for AβO detection, utilizing a novel custom-designed AβO-selective fluorophore, named O-SLM. This innovative approach achieves a sensitivity nearly tenfold greater than that of a comparative ELISA kit, with a detection limit as low as 25 fM. The assay requires minimal sample volumes and streamlines the protocol by omitting detection antibodies. It effectively distinguishes AD patients from healthy individuals through serum AβO quantification, demonstrating the versatility of our ultra-sensitive assay across various biomarkers.

AB - The rising prevalence of Alzheimer's disease (AD) highlights an urgent need for ultra-sensitive diagnostic tools that facilitate early detection and intervention. Soluble Aβ oligomers (AβOs) have emerged as a key focus due to their specificity for AD pathology. This study introduces a magnetic nanoplatform-based ultrasensitive diagnostic assay for AβO detection, utilizing a novel custom-designed AβO-selective fluorophore, named O-SLM. This innovative approach achieves a sensitivity nearly tenfold greater than that of a comparative ELISA kit, with a detection limit as low as 25 fM. The assay requires minimal sample volumes and streamlines the protocol by omitting detection antibodies. It effectively distinguishes AD patients from healthy individuals through serum AβO quantification, demonstrating the versatility of our ultra-sensitive assay across various biomarkers.

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Direct and selective nanosensor for amyloid-β oligomers in serum for Alzheimer's disease diagnosis

Abstract

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