Direct analysis of alkaloid profiling in plant tissue by using matrix-assisted laser desorption/ionization mass spectrometry

Wei Wu, Zhitao LIANG, Zhongzhen ZHAO, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

A method for the direct determination of alkaloid profiling in plant tissues by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was developed. The alkaloid profiles of the herbs were obtained without the need for complicated sample preparation. Experimental results demonstrated that the direct MALDI-TOFMS analysis allowed rapid and reliable characterization of the components in plant tissues. Four commonly used Chinese medicinal herbs were studied, including Aconitum Carmichaeli Debx. (Fuzi in Chinese) and Processed Fuzi, for herb differentiation and explanation of the significant difference in their toxicities. The direct analysis method proved valuable for the preliminary study of plant component profiles. The rapid collection of information from the direct analysis on plant tissues could be valuable for supporting the discovery of new compounds and for the quality control of medicinal herbs.

Original languageEnglish
Pages (from-to)58-69
Number of pages12
JournalJournal of Mass Spectrometry
Volume42
Issue number1
DOIs
Publication statusPublished - Jan 2007

Scopus Subject Areas

  • Spectroscopy

User-Defined Keywords

  • Alkaloids
  • Component profiling
  • MALDI-TOFMS direct analysis
  • Plant tissue

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