DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent bio-samples

Isabell Whiteley*, Chenchen Song, Glenn A. Howe, Thomas Knöpfel, Christopher J. Rowlands

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

1 Citation (Scopus)


A targeted imaging system has been developed for applications requiring recording from stationary samples at high spatiotemporal resolutions. It works by illuminating regions of interest in rapid sequence, and recording the signal from the whole field of view onto a single photodetector. It can be implemented at low cost on an existing microscope without compromising existing functionality. The system is characterized in terms of speed, spatial resolution, and tissue penetration depth, before being used to record individual action potentials from ASAP-3 expressing neurons in an ex vivo mouse brain slice preparation.

Original languageEnglish
Pages (from-to)2565-2575
Number of pages11
JournalBiomedical Optics Express
Issue number6
Early online date8 May 2023
Publication statusPublished - 1 Jun 2023

Scopus Subject Areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


Dive into the research topics of 'DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent bio-samples'. Together they form a unique fingerprint.

Cite this