DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent bio-samples

Isabell Whiteley; Chenchen Song; Glenn A. Howe; Thomas Knöpfel; Christopher J. Rowlands

doi:10.1364/BOE.486507

DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent bio-samples

Isabell Whiteley^*, Chenchen Song, Glenn A. Howe, Thomas Knöpfel, Christopher J. Rowlands

^*Corresponding author for this work

Department of Physics

Research output: Contribution to journal › Journal article › peer-review

1 Citation (Scopus)

Abstract

A targeted imaging system has been developed for applications requiring recording from stationary samples at high spatiotemporal resolutions. It works by illuminating regions of interest in rapid sequence, and recording the signal from the whole field of view onto a single photodetector. It can be implemented at low cost on an existing microscope without compromising existing functionality. The system is characterized in terms of speed, spatial resolution, and tissue penetration depth, before being used to record individual action potentials from ASAP-3 expressing neurons in an ex vivo mouse brain slice preparation.

Original language	English
Pages (from-to)	2565-2575
Number of pages	11
Journal	Biomedical Optics Express
Volume	14
Issue number	6
Early online date	8 May 2023
DOIs	https://doi.org/10.1364/BOE.486507
Publication status	Published - 1 Jun 2023

Scopus Subject Areas

Biotechnology
Atomic and Molecular Physics, and Optics

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1364/BOE.486507

Cite this

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AU - Rowlands, Christopher J.

PY - 2023/6/1

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AB - A targeted imaging system has been developed for applications requiring recording from stationary samples at high spatiotemporal resolutions. It works by illuminating regions of interest in rapid sequence, and recording the signal from the whole field of view onto a single photodetector. It can be implemented at low cost on an existing microscope without compromising existing functionality. The system is characterized in terms of speed, spatial resolution, and tissue penetration depth, before being used to record individual action potentials from ASAP-3 expressing neurons in an ex vivo mouse brain slice preparation.

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DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent bio-samples

Abstract

Scopus Subject Areas

UN SDGs

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