TY - JOUR
T1 - Dingxin recipe prevents ischemia/reperfusion- Induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses
AU - Jia, Yu Hua
AU - Zhang, Yun Xian
AU - Li, Li Jun
AU - Liu, Ya Wei
AU - Li, Chun Hua
AU - Fu, Xiu Qiong
AU - Zeng, Ping
AU - Wu, Wei Kang
AU - Sun, Xue Gang
N1 - Funding Information:
©The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag Berlin Heidelberg 2012 Supported by the National Natural Science Foundation of China (No. 30572435 and No. 81072776) and Baiyun District Science and Technology Program (No. 2010-KZ-39) 1. The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou (510515), China; 2. Fuda Cancer Hospital, Guangzhou (510300), China; 3. Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou (510120), China; 4. The Institute of Integrated Traditional Chinese and Western Medicine, Sun Yat-sen University, Guangzhou (510120), China Correspondence to: Prof. SUN Xue-gang, Tel: 86-20-61648541, 86-013632102936, Fax: 86-20-61648245, E-mail: sxg_ [email protected] DOI: 10.1007/s11655-012-0998-x
PY - 2012/2
Y1 - 2012/2
N2 - Objective: To identify the underlying mechanisms of the protective effects of Dingxin Recipe (DXR), a Chinese compound prescription that has been used clinically in China for more than 20 years, on ischemia/reperfusion (I/R)-induced arrhythmias in rat model. Methods: A total of 30 rats were randomly divided into three groups: sham group, I/R group, and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days, while rats in the sham and I/R groups were administrated with normal saline. Arrhythmias were introduced by I/R and electrocardiograms (ECG) were recorded. Two-dimensional (2-D) polyacrylamide gel electrophoresis and matrixassisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify differentially expressed proteins. Immunohistochemistry, real-time quantitative polymerase chain reaction (RQPCR), Western blot, and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins obtained in the above experiments. Results: DXR significantly reduced the incidence and mean duration of ventricular tachycardia and ventricular fibrillation and dramatically decreased the mortality, as well as arrhythmia score, compared with those of the I/R group. Among successfully identified proteins, prohibitin (PHB) and heart fatty acid binding protein (hFABP) were up-regulated in DXR-pretreated I/R rats compared with those of the I/R rats. In addition, compared with the I/R group, the level of glutathione (GSH) was elevated accompanied by reduced expressions of interleukin-6 (IL-6) and neutrophil infiltration in I/R rats with DXR pretreatment. Conclusions: DXR could alleviate I/R-induced arrhythmias, which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore, up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.
AB - Objective: To identify the underlying mechanisms of the protective effects of Dingxin Recipe (DXR), a Chinese compound prescription that has been used clinically in China for more than 20 years, on ischemia/reperfusion (I/R)-induced arrhythmias in rat model. Methods: A total of 30 rats were randomly divided into three groups: sham group, I/R group, and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days, while rats in the sham and I/R groups were administrated with normal saline. Arrhythmias were introduced by I/R and electrocardiograms (ECG) were recorded. Two-dimensional (2-D) polyacrylamide gel electrophoresis and matrixassisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify differentially expressed proteins. Immunohistochemistry, real-time quantitative polymerase chain reaction (RQPCR), Western blot, and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins obtained in the above experiments. Results: DXR significantly reduced the incidence and mean duration of ventricular tachycardia and ventricular fibrillation and dramatically decreased the mortality, as well as arrhythmia score, compared with those of the I/R group. Among successfully identified proteins, prohibitin (PHB) and heart fatty acid binding protein (hFABP) were up-regulated in DXR-pretreated I/R rats compared with those of the I/R rats. In addition, compared with the I/R group, the level of glutathione (GSH) was elevated accompanied by reduced expressions of interleukin-6 (IL-6) and neutrophil infiltration in I/R rats with DXR pretreatment. Conclusions: DXR could alleviate I/R-induced arrhythmias, which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore, up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.
KW - Arrhythmias
KW - Dingxin Recipe
KW - Inflammation
KW - Ischemia/reperfusion
KW - Prohibitin
KW - Proteomics
UR - https://www.scopus.com/pages/publications/84862741915
U2 - 10.1007/s11655-012-0998-x
DO - 10.1007/s11655-012-0998-x
M3 - Journal article
C2 - 22311408
AN - SCOPUS:84862741915
SN - 1672-0415
VL - 18
SP - 120
EP - 129
JO - Chinese Journal of Integrative Medicine
JF - Chinese Journal of Integrative Medicine
IS - 2
ER -