Dingxin recipe prevents ischemia/reperfusion- Induced arrhythmias via up-regulating prohibitin and suppressing inflammatory responses

Yu Hua Jia, Yun Xian Zhang, Li Jun Li, Ya Wei Liu, Chun Hua Li, Xiu Qiong Fu, Ping Zeng, Wei Kang Wu, Xue Gang Sun*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

13 Citations (Scopus)


Objective: To identify the underlying mechanisms of the protective effects of Dingxin Recipe (DXR), a Chinese compound prescription that has been used clinically in China for more than 20 years, on ischemia/reperfusion (I/R)-induced arrhythmias in rat model. Methods: A total of 30 rats were randomly divided into three groups: sham group, I/R group, and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days, while rats in the sham and I/R groups were administrated with normal saline. Arrhythmias were introduced by I/R and electrocardiograms (ECG) were recorded. Two-dimensional (2-D) polyacrylamide gel electrophoresis and matrixassisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify differentially expressed proteins. Immunohistochemistry, real-time quantitative polymerase chain reaction (RQPCR), Western blot, and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins obtained in the above experiments. Results: DXR significantly reduced the incidence and mean duration of ventricular tachycardia and ventricular fibrillation and dramatically decreased the mortality, as well as arrhythmia score, compared with those of the I/R group. Among successfully identified proteins, prohibitin (PHB) and heart fatty acid binding protein (hFABP) were up-regulated in DXR-pretreated I/R rats compared with those of the I/R rats. In addition, compared with the I/R group, the level of glutathione (GSH) was elevated accompanied by reduced expressions of interleukin-6 (IL-6) and neutrophil infiltration in I/R rats with DXR pretreatment. Conclusions: DXR could alleviate I/R-induced arrhythmias, which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore, up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.

Original languageEnglish
Pages (from-to)120-129
Number of pages10
JournalChinese Journal of Integrative Medicine
Issue number2
Publication statusPublished - Feb 2012

Scopus Subject Areas

  • Complementary and alternative medicine
  • Pharmacology (medical)

User-Defined Keywords

  • Arrhythmias
  • Dingxin Recipe
  • Inflammation
  • Ischemia/reperfusion
  • Prohibitin
  • Proteomics


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