Differentiation of Lycium barbarum from its related Lycium species using random amplified polymorphic DNA

K. Y.B. Zhang; H. W. Leung; H. W. Yeung; R. N.S. Wong

doi:10.1055/s-2001-14310

Differentiation of Lycium barbarum from its related Lycium species using random amplified polymorphic DNA

K. Y.B. Zhang, H. W. Leung, H. W. Yeung, R. N.S. Wong^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

50 Citations (Scopus)

Abstract

The RAPD (random amplified polymorphic DNA) technique was applied for the first time to distinguish Lycium barbarum from other closely related species of the same genus. In this study, eight samples were collected, including five species, two varieties and one cultivated variety. A total of fifty arbitrary primers were used in the RAPD analysis. Distinctive DNA fingerprints corresponding to different Lycium species were successfully obtained from ten primers. Similarity index (S.I.) analysis revealed that the values are higher between intraspecies than interspecies. These results confirmed that the RAPD technique can be employed for distinguishing closely related species of Lycium.

Original language	English
Pages (from-to)	379-381
Number of pages	3
Journal	Planta Medica
Volume	67
Issue number	4
DOIs	https://doi.org/10.1055/s-2001-14310
Publication status	Published - 2001

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title = "Differentiation of Lycium barbarum from its related Lycium species using random amplified polymorphic DNA",

abstract = "The RAPD (random amplified polymorphic DNA) technique was applied for the first time to distinguish Lycium barbarum from other closely related species of the same genus. In this study, eight samples were collected, including five species, two varieties and one cultivated variety. A total of fifty arbitrary primers were used in the RAPD analysis. Distinctive DNA fingerprints corresponding to different Lycium species were successfully obtained from ten primers. Similarity index (S.I.) analysis revealed that the values are higher between intraspecies than interspecies. These results confirmed that the RAPD technique can be employed for distinguishing closely related species of Lycium.",

author = "Zhang, {K. Y.B.} and Leung, {H. W.} and Yeung, {H. W.} and Wong, {R. N.S.}",

note = "The work was supported by an Industrial Support Fund (AF/249/97), from the Industry Department of the Hong Kong SAR.",

year = "2001",

doi = "10.1055/s-2001-14310",

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journal = "Planta Medica",

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T1 - Differentiation of Lycium barbarum from its related Lycium species using random amplified polymorphic DNA

AU - Zhang, K. Y.B.

AU - Leung, H. W.

AU - Yeung, H. W.

AU - Wong, R. N.S.

N1 - The work was supported by an Industrial Support Fund (AF/249/97), from the Industry Department of the Hong Kong SAR.

PY - 2001

Y1 - 2001

N2 - The RAPD (random amplified polymorphic DNA) technique was applied for the first time to distinguish Lycium barbarum from other closely related species of the same genus. In this study, eight samples were collected, including five species, two varieties and one cultivated variety. A total of fifty arbitrary primers were used in the RAPD analysis. Distinctive DNA fingerprints corresponding to different Lycium species were successfully obtained from ten primers. Similarity index (S.I.) analysis revealed that the values are higher between intraspecies than interspecies. These results confirmed that the RAPD technique can be employed for distinguishing closely related species of Lycium.

AB - The RAPD (random amplified polymorphic DNA) technique was applied for the first time to distinguish Lycium barbarum from other closely related species of the same genus. In this study, eight samples were collected, including five species, two varieties and one cultivated variety. A total of fifty arbitrary primers were used in the RAPD analysis. Distinctive DNA fingerprints corresponding to different Lycium species were successfully obtained from ten primers. Similarity index (S.I.) analysis revealed that the values are higher between intraspecies than interspecies. These results confirmed that the RAPD technique can be employed for distinguishing closely related species of Lycium.

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M3 - Journal article

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SN - 0032-0943

VL - 67

SP - 379

EP - 381

JO - Planta Medica

JF - Planta Medica

IS - 4

ER -

Differentiation of Lycium barbarum from its related Lycium species using random amplified polymorphic DNA

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