Differentiation of herbs linked to "Chinese herb nephropathy" from the liquid chromatographic determination of aristolochic acids

Wan Chan; Ka Man Hui; Wing Tat Poon; Kim Chung Lee; Zongwei Cai

doi:10.1016/j.aca.2006.03.008

Differentiation of herbs linked to "Chinese herb nephropathy" from the liquid chromatographic determination of aristolochic acids

Wan Chan, Ka Man Hui, Wing Tat Poon, Kim Chung Lee, Zongwei Cai^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

34 Citations (Scopus)

Abstract

A HPLC method was developed and applied to analyze aristolochic acids (AA-I and AA-II) in Chinese medicinal herbs. The herb samples were extracted by using ultrasonication with the extraction efficiency of better than 82%. Extracts were then filtered and injected onto a C18 column eluting under a gradient program using methanol and water-containing 0.5% acetic acid. The method with the detection limits of 1.33 ng for AA-I and 7.29 ng for AA-II per injection was successfully applied for the analysis of traditional Chinese medicine (TCM) and related products and differentiation of Chinese medicinal herbs that have previously been misused and caused toxicological effects. The developed protocol provided an example that analysis of selected component markers could serve for health security and quality control of TCM consumption.

Original language	English
Pages (from-to)	112-116
Number of pages	5
Journal	Analytica Chimica Acta
Volume	576
Issue number	1
DOIs	https://doi.org/10.1016/j.aca.2006.03.008
Publication status	Published - 18 Aug 2006

Scopus Subject Areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

User-Defined Keywords

Aristolochic acids
Chinese herb nephropathy
Chinese herbal medicine
HPLC

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10.1016/j.aca.2006.03.008

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@article{051de2f865de46baaca5cc675ede82e1,

title = "Differentiation of herbs linked to {"}Chinese herb nephropathy{"} from the liquid chromatographic determination of aristolochic acids",

abstract = "A HPLC method was developed and applied to analyze aristolochic acids (AA-I and AA-II) in Chinese medicinal herbs. The herb samples were extracted by using ultrasonication with the extraction efficiency of better than 82%. Extracts were then filtered and injected onto a C18 column eluting under a gradient program using methanol and water-containing 0.5% acetic acid. The method with the detection limits of 1.33 ng for AA-I and 7.29 ng for AA-II per injection was successfully applied for the analysis of traditional Chinese medicine (TCM) and related products and differentiation of Chinese medicinal herbs that have previously been misused and caused toxicological effects. The developed protocol provided an example that analysis of selected component markers could serve for health security and quality control of TCM consumption.",

keywords = "Aristolochic acids, Chinese herb nephropathy, Chinese herbal medicine, HPLC",

author = "Wan Chan and Hui, {Ka Man} and Poon, {Wing Tat} and Lee, {Kim Chung} and Zongwei Cai",

note = "Funding Information: The authors would like to thank the Faculty Research Grant from the Hong Kong Baptist University (FRG04-05/II-35) and the Research Grant Council, University Grants Committee of Hong Kong (HKBU2154/04M) for their financial supports for this study.",

year = "2006",

month = aug,

day = "18",

doi = "10.1016/j.aca.2006.03.008",

language = "English",

volume = "576",

pages = "112--116",

journal = "Analytica Chimica Acta",

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T1 - Differentiation of herbs linked to "Chinese herb nephropathy" from the liquid chromatographic determination of aristolochic acids

AU - Chan, Wan

AU - Hui, Ka Man

AU - Poon, Wing Tat

AU - Lee, Kim Chung

AU - Cai, Zongwei

N1 - Funding Information: The authors would like to thank the Faculty Research Grant from the Hong Kong Baptist University (FRG04-05/II-35) and the Research Grant Council, University Grants Committee of Hong Kong (HKBU2154/04M) for their financial supports for this study.

PY - 2006/8/18

Y1 - 2006/8/18

N2 - A HPLC method was developed and applied to analyze aristolochic acids (AA-I and AA-II) in Chinese medicinal herbs. The herb samples were extracted by using ultrasonication with the extraction efficiency of better than 82%. Extracts were then filtered and injected onto a C18 column eluting under a gradient program using methanol and water-containing 0.5% acetic acid. The method with the detection limits of 1.33 ng for AA-I and 7.29 ng for AA-II per injection was successfully applied for the analysis of traditional Chinese medicine (TCM) and related products and differentiation of Chinese medicinal herbs that have previously been misused and caused toxicological effects. The developed protocol provided an example that analysis of selected component markers could serve for health security and quality control of TCM consumption.

AB - A HPLC method was developed and applied to analyze aristolochic acids (AA-I and AA-II) in Chinese medicinal herbs. The herb samples were extracted by using ultrasonication with the extraction efficiency of better than 82%. Extracts were then filtered and injected onto a C18 column eluting under a gradient program using methanol and water-containing 0.5% acetic acid. The method with the detection limits of 1.33 ng for AA-I and 7.29 ng for AA-II per injection was successfully applied for the analysis of traditional Chinese medicine (TCM) and related products and differentiation of Chinese medicinal herbs that have previously been misused and caused toxicological effects. The developed protocol provided an example that analysis of selected component markers could serve for health security and quality control of TCM consumption.

KW - Aristolochic acids

KW - Chinese herb nephropathy

KW - Chinese herbal medicine

KW - HPLC

UR - http://www.scopus.com/inward/record.url?scp=33746867162&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2006.03.008

DO - 10.1016/j.aca.2006.03.008

M3 - Journal article

AN - SCOPUS:33746867162

SN - 0003-2670

VL - 576

SP - 112

EP - 116

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1

ER -

Differentiation of herbs linked to "Chinese herb nephropathy" from the liquid chromatographic determination of aristolochic acids

Abstract

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