Differential regulation of hepatic SH3 domain binding kinase 1 (SBK1) expression in mouse and goldfish

Miaojia Hang, Margaret Chui Ling Tse, Brian Pak Shing Pang, Xinyi Bi, Fanming Jin, Chi Wai Lee, Anderson O.L. Wong, Chi Bun Chan*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review


SH3 domain binding kinase 1 (SBK1) is a serine/threonine kinase that belongs to the new kinase family (NFK) with limited information on its function. Previous studies reported that SBK1 plays a role in memory formation, lipid metabolism, and cancer cell progression. Nevertheless, the regulatory mechanism of Sbk1 expression in various tissues remains unknown. We report here that Sbk1 expression in mouse hepatocytes was downregulated by glucocorticoid, whereas saturated and unsaturated fatty acids were stimulators of Sbk1 expression. The regulatory role of glucocorticoid and fatty acid was further confirmed by the Sbk1 promoter assay, which aligned with the presence of several glucocorticoid-response elements (GRE) and peroxisome proliferator responsive elements (PPRE) in the mouse Sbk1 promoter. The inhibitory effect of glucocorticoids on hepatic Sbk1 expression and protein content could also be demonstrated in vivo after prednisolone injection. Moreover, the expression of SBK1 in goldfish (gfSBK1) was also sensitive to glucocorticoid suppression as their mouse orthologues. In contrast, insulin had a differential action on SBK1 expression that it promoted the expression of all SBK1 isoforms in the goldfish hepatocytes but inhibited Sbk1 expression in the mouse hepatocytes. Together, our findings indicate that SBK1 expression is hormone- and nutrient-sensitive with a species-specific response.

Original languageEnglish
Article number114372
Number of pages8
JournalGeneral and Comparative Endocrinology
Early online date29 Aug 2023
Publication statusPublished - 1 Dec 2023

Scopus Subject Areas

  • Endocrinology
  • Animal Science and Zoology

User-Defined Keywords

  • Hormone
  • Liver
  • SBK1


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