Differential-pulse polarographic micro-determination of amino acids via in situ generation of dithiocarbamates

Kwok Keung SHIU*, Wing Hong CHAN, Albert W M LEE, Wai Cheong Wong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Amino acids underwent in situ derivatization with carbon disulfide in the presence of triethylamine at room temperature to yield the corresponding dithiocarbamate derivatives, which were then determined in aqueous media by differential-pulse polarography. The dithiocarbamate derivative of an amino acid usually showed a characteristic cathodic peak in the range between -0.51 and -0.61 V (versus Ag-;AgCl). Calibration graphs obtained by plotting the measured polarographic peak current of the dithiocarbamate derivative versus the concentration of the amino acid undergoing derivatization were rectilinear over the range 1.6-20 μmol l-1. For the micro-determination of amino acids, the method was found to be precise, and the detection limit was calculated to be 1 μg.

Original languageEnglish
Pages (from-to)869-872
Number of pages4
JournalThe Analyst
Volume118
Issue number7
DOIs
Publication statusPublished - 1993

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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