Differential gene expression in Escherichia coli during aerosolization from liquid suspension

Tsz Wai Ng, Margaret Ip, Christopher Y.H. Chao, Julian Wei Tang, Keng Po Lai, Sau Chung Fu, Wing Tong Leung, Ka Man LAI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Comparative transcriptome analysis was used to determine the differentially expressed genes in Escherichia coli during aerosolization from liquid suspension. Isogenic mutant studies were then used to examine the potential part played by some of these genes in bacterial survival in the air. Bioaerosols were sampled after 3 min of nebulization, which aerosolized the bacteria from the liquid suspension to an aerosol chamber (A0), and after further 30 min of airborne suspension in the chamber (A30). Bacteria at A0 showed 65 differentially expressed genes (30 downregulated and 35 upregulated) as compared to the original bacteria in the nebulizer. Droplet evaporation models predicted a drop in temperature in the bioaerosols, which coincides with the change in the expression of cold shock protein genes—cspB and cspG in the bacteria. The most notable group of differentially expressed genes was sorbitol transport and metabolism genes (srlABDEMR). Other genes associated with osmotic stress, nutrient limitation, DNA damage, and other stresses were differentially expressed in the bacteria at A0. After further airborne suspension, one gene (ypfM, which encodes a hypothetical protein with unknown function) was downregulated in the bacteria at A30 as compared to those at A0. Finally, isogenic mutants with either the dps or srlA gene deleted (both genes were upregulated at A0) had lower survival than the parental strain, which is a sign of their potential ability to protect the bacteria in the air.

Original languageEnglish
Pages (from-to)6257-6267
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume102
Issue number14
DOIs
Publication statusPublished - 1 Jul 2018

Scopus Subject Areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

User-Defined Keywords

  • Airborne bacteria
  • RNA-seq
  • Stress response
  • Transcriptome analysis

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