Abstract
Objectives: This study evaluated the effects of diethyl aminoethyl hexonoate (DA-6) on the accumulation of chicoric acid in Echinacea purpurea plants.
Methods and Results: Plantlets of a clone were cultured in vitro for 120 days on Murashige and Skoog's medium supplemented with 0 (control), 0.08, 0.16, 0.32, and 0.64 mg/L DA-6. Results showed that the concentration of chicoric acid of plantlets growing on medium containing 0.32 mg/L DA-6 was found to be 3.65 mg/g in the aerial part, 68% higher than that in the control group of 2.17 mg/g; and 6.18 mg/g in the underground part, 19% higher than that in the control group of 5.18 mg/g. Plants grown in pots were treated before the formation of flower buds with 10 drops of 0.32 mg/L DA-6 solution (especially at the stem apex) and 10 drops of pure water as the control. The treatment was repeated four times with an interval of 3 days. The leaf length of the treated plants increased to 11.85 cm in 20 days, 68% longer than that in the control group of 7.05 cm. Field-grown plants were treated by spraying of the foliage with DA-6 solution three times at the concentrations of 0 (control), 2.5, 5, 10, and 50 mg/L; the treatment interval was 15 days. Results of the treatment effects were recorded 1 month later. It was found that plants treated with 2.5 and 5 mg/L DA-6 developed 13 and 23 inflorescences/plant, respectively, while the plants in the control group developed only 7 inflorescences/plant. Treating with 5 and 10 mg/L DA-6 increased the total chicoric acid content to 155.55 and 164.60 mg/plant, 171% and 187% higher than that in the control group of 57.40 mg/plant, respectively. A higher DA-6 concentration of 50 mg/L also increased the content of chicoric acid in the plants (the highest amount of 190.20 mg/plant was detected).
Conclusions: To sum up, employing a certain concentration of DA-6 may promote the development and enhance the accumulation of chicoric acid in E. purpurea plants.
Methods and Results: Plantlets of a clone were cultured in vitro for 120 days on Murashige and Skoog's medium supplemented with 0 (control), 0.08, 0.16, 0.32, and 0.64 mg/L DA-6. Results showed that the concentration of chicoric acid of plantlets growing on medium containing 0.32 mg/L DA-6 was found to be 3.65 mg/g in the aerial part, 68% higher than that in the control group of 2.17 mg/g; and 6.18 mg/g in the underground part, 19% higher than that in the control group of 5.18 mg/g. Plants grown in pots were treated before the formation of flower buds with 10 drops of 0.32 mg/L DA-6 solution (especially at the stem apex) and 10 drops of pure water as the control. The treatment was repeated four times with an interval of 3 days. The leaf length of the treated plants increased to 11.85 cm in 20 days, 68% longer than that in the control group of 7.05 cm. Field-grown plants were treated by spraying of the foliage with DA-6 solution three times at the concentrations of 0 (control), 2.5, 5, 10, and 50 mg/L; the treatment interval was 15 days. Results of the treatment effects were recorded 1 month later. It was found that plants treated with 2.5 and 5 mg/L DA-6 developed 13 and 23 inflorescences/plant, respectively, while the plants in the control group developed only 7 inflorescences/plant. Treating with 5 and 10 mg/L DA-6 increased the total chicoric acid content to 155.55 and 164.60 mg/plant, 171% and 187% higher than that in the control group of 57.40 mg/plant, respectively. A higher DA-6 concentration of 50 mg/L also increased the content of chicoric acid in the plants (the highest amount of 190.20 mg/plant was detected).
Conclusions: To sum up, employing a certain concentration of DA-6 may promote the development and enhance the accumulation of chicoric acid in E. purpurea plants.
| Original language | English |
|---|---|
| Journal | Basic and Clinical Pharmacology and Toxicology |
| Volume | 134 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 22 Nov 2019 |
