Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H2O2 through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity

Chi Chun Fong, Yaou Zhang, Qi Zhang, Chi Hung Tzang, David W F FONG, Rodulf S.S. Wu, Mengsu Yang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

In this study, the effect of dexamethasone, a synthetic glucocorticoid, on H2O2 stimulated murine RAW264.7 macrophages was investigated. It was found that dexamethasone protected the cells from apoptosis induced by H2O2. A cDNA microarray, which consists of 1000 genes selected from a mouse clone set provided from NIA, was used to study the gene expression profiles involved in the protective effect. Our data show that dexamethasone exerts the anti-apoptosis function by changing the expression patterns of many genes involved inhibiting the up-regulation of apoptosis promoting genes and the down-regulation of cell cycle stimulating genes as well as keeping the up-regulation of cell survival related genes. Our study also revealed that dexamethasone protects RAW264.7 macrophages from H2O2 induced apoptosis through blocking nuclear factor-kappa B (NF-κB) activity.

Original languageEnglish
Pages (from-to)16-28
Number of pages13
JournalToxicology
Volume236
Issue number1-2
DOIs
Publication statusPublished - 1 Jul 2007

Scopus Subject Areas

  • Toxicology

User-Defined Keywords

  • Dexamethasone
  • Gene expression
  • Hydrogen peroxide
  • NF-κB activity
  • RAW264.7 macrophages

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