Developmental expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body of the rat

Izaskun Elezgarai; Rocío Benítez; José María Mateos; Esther Lázaro; Alexandra Osorio; Jon Jatsu Azkue; Aurora Bilbao; Kurt Lingenhoehl; Herman Van Der Putten; David R. Hampson; Rainer Kuhn; Thomas Knöpfel; Pedro Grandes

doi:10.1002/(SICI)1096-9861(19990830)411:3<431::AID-CNE6>3.0.CO;2-R

Developmental expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body of the rat

Izaskun Elezgarai, Rocío Benítez, José María Mateos, Esther Lázaro, Alexandra Osorio, Jon Jatsu Azkue, Aurora Bilbao, Kurt Lingenhoehl, Herman Van Der Putten, David R. Hampson, Rainer Kuhn, Thomas Knöpfel, Pedro Grandes^*

^*Corresponding author for this work

Department of Physics

Research output: Contribution to journal › Journal article › peer-review

34 Citations (Scopus)

Abstract

A preembedding immunocytochemical method for light microscopy was used to study the postnatal development of expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body (MNTB) of the rat. Immunoreactivity for mGluR4a was localized in axonal endings wrapping the principal globular neurons in MNTB, known as calyces of Held. The percentage of calyces of Held immunoreactive for mGluR4a increased progressively from postnatal day 3 (PND3), showing the highest density of labeled calyces by PND9. From this postnatal age on, a gradual reduction in the number of mGluR4a-immunopositive calyces of Held was observed, reaching the lowest level of labeled profiles in adult tissue. The developmental expression of mGluR4a in calyces of Held correlates well with previous studies in young animals showing a modulation of synaptic neurotransmission by group III mGluRs in these giant excitatory synapses made on MNTB principal neurons. All these observations together suggest that the expression of mGluR4a mainly between PND7 and PND12 might be relevant to the maturation and modulation of synaptic transmission at the calyces of Held.

Original language	English
Pages (from-to)	431-440
Number of pages	10
Journal	Journal of Comparative Neurology
Volume	411
Issue number	3
DOIs	https://doi.org/10.1002/(SICI)1096-9861(19990830)411:3<431::AID-CNE6>3.0.CO;2-R
Publication status	Published - 30 Aug 1999

User-Defined Keywords

Auditory system
Calyces of Held
Excitatory amino acid receptor
G-proteins
Light microscopy
Preembedding immunocytochemistry

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10.1002/(SICI)1096-9861(19990830)411:3<431::AID-CNE6>3.0.CO;2-R

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Elezgarai, I., Benítez, R., Mateos, J. M., Lázaro, E., Osorio, A., Azkue, J. J., Bilbao, A., Lingenhoehl, K., Van Der Putten, H., Hampson, D. R., Kuhn, R., Knöpfel, T., & Grandes, P. (1999). Developmental expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body of the rat. Journal of Comparative Neurology, 411(3), 431-440. https://doi.org/10.1002/(SICI)1096-9861(19990830)411:3<431::AID-CNE6>3.0.CO;2-R

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title = "Developmental expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body of the rat",

abstract = "A preembedding immunocytochemical method for light microscopy was used to study the postnatal development of expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body (MNTB) of the rat. Immunoreactivity for mGluR4a was localized in axonal endings wrapping the principal globular neurons in MNTB, known as calyces of Held. The percentage of calyces of Held immunoreactive for mGluR4a increased progressively from postnatal day 3 (PND3), showing the highest density of labeled calyces by PND9. From this postnatal age on, a gradual reduction in the number of mGluR4a-immunopositive calyces of Held was observed, reaching the lowest level of labeled profiles in adult tissue. The developmental expression of mGluR4a in calyces of Held correlates well with previous studies in young animals showing a modulation of synaptic neurotransmission by group III mGluRs in these giant excitatory synapses made on MNTB principal neurons. All these observations together suggest that the expression of mGluR4a mainly between PND7 and PND12 might be relevant to the maturation and modulation of synaptic transmission at the calyces of Held.",

keywords = "Auditory system, Calyces of Held, Excitatory amino acid receptor, G-proteins, Light microscopy, Preembedding immunocytochemistry",

author = "Izaskun Elezgarai and Roc{\'i}o Ben{\'i}tez and Mateos, {Jos{\'e} Mar{\'i}a} and Esther L{\'a}zaro and Alexandra Osorio and Azkue, {Jon Jatsu} and Aurora Bilbao and Kurt Lingenhoehl and {Van Der Putten}, Herman and Hampson, {David R.} and Rainer Kuhn and Thomas Kn{\"o}pfel and Pedro Grandes",

note = "Publisher copyright: {\textcopyright} 1999 Wiley-Liss, Inc.",

year = "1999",

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doi = "10.1002/(SICI)1096-9861(19990830)411:3<431::AID-CNE6>3.0.CO;2-R",

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Elezgarai, I, Benítez, R, Mateos, JM, Lázaro, E, Osorio, A, Azkue, JJ, Bilbao, A, Lingenhoehl, K, Van Der Putten, H, Hampson, DR, Kuhn, R, Knöpfel, T & Grandes, P 1999, 'Developmental expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body of the rat', Journal of Comparative Neurology, vol. 411, no. 3, pp. 431-440. https://doi.org/10.1002/(SICI)1096-9861(19990830)411:3<431::AID-CNE6>3.0.CO;2-R

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T1 - Developmental expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body of the rat

AU - Elezgarai, Izaskun

AU - Benítez, Rocío

AU - Mateos, José María

AU - Lázaro, Esther

AU - Osorio, Alexandra

AU - Azkue, Jon Jatsu

AU - Bilbao, Aurora

AU - Lingenhoehl, Kurt

AU - Van Der Putten, Herman

AU - Hampson, David R.

AU - Kuhn, Rainer

AU - Knöpfel, Thomas

AU - Grandes, Pedro

PY - 1999/8/30

Y1 - 1999/8/30

N2 - A preembedding immunocytochemical method for light microscopy was used to study the postnatal development of expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body (MNTB) of the rat. Immunoreactivity for mGluR4a was localized in axonal endings wrapping the principal globular neurons in MNTB, known as calyces of Held. The percentage of calyces of Held immunoreactive for mGluR4a increased progressively from postnatal day 3 (PND3), showing the highest density of labeled calyces by PND9. From this postnatal age on, a gradual reduction in the number of mGluR4a-immunopositive calyces of Held was observed, reaching the lowest level of labeled profiles in adult tissue. The developmental expression of mGluR4a in calyces of Held correlates well with previous studies in young animals showing a modulation of synaptic neurotransmission by group III mGluRs in these giant excitatory synapses made on MNTB principal neurons. All these observations together suggest that the expression of mGluR4a mainly between PND7 and PND12 might be relevant to the maturation and modulation of synaptic transmission at the calyces of Held.

AB - A preembedding immunocytochemical method for light microscopy was used to study the postnatal development of expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body (MNTB) of the rat. Immunoreactivity for mGluR4a was localized in axonal endings wrapping the principal globular neurons in MNTB, known as calyces of Held. The percentage of calyces of Held immunoreactive for mGluR4a increased progressively from postnatal day 3 (PND3), showing the highest density of labeled calyces by PND9. From this postnatal age on, a gradual reduction in the number of mGluR4a-immunopositive calyces of Held was observed, reaching the lowest level of labeled profiles in adult tissue. The developmental expression of mGluR4a in calyces of Held correlates well with previous studies in young animals showing a modulation of synaptic neurotransmission by group III mGluRs in these giant excitatory synapses made on MNTB principal neurons. All these observations together suggest that the expression of mGluR4a mainly between PND7 and PND12 might be relevant to the maturation and modulation of synaptic transmission at the calyces of Held.

KW - Auditory system

KW - Calyces of Held

KW - Excitatory amino acid receptor

KW - G-proteins

KW - Light microscopy

KW - Preembedding immunocytochemistry

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U2 - 10.1002/(SICI)1096-9861(19990830)411:3<431::AID-CNE6>3.0.CO;2-R

DO - 10.1002/(SICI)1096-9861(19990830)411:3<431::AID-CNE6>3.0.CO;2-R

M3 - Journal article

C2 - 10413777

AN - SCOPUS:0345085693

SN - 0021-9967

VL - 411

SP - 431

EP - 440

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

IS - 3

ER -

Developmental expression of the group III metabotropic glutamate receptor mGluR4a in the medial nucleus of the trapezoid body of the rat

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