TY - JOUR
T1 - Development of multiplex nucleic acid sequence-based amplification for detection of human respiratory tract viruses
AU - Lau, Lok Ting
AU - Feng, Xiao Yan
AU - Lam, Tsz Yan
AU - Hui, Hung Kit
AU - Yu, Albert Cheung Hoi
N1 - Copyright:
© 2010 Elsevier B.V. All rights reserved.
PY - 2010/9
Y1 - 2010/9
N2 - A group of common lower respiratory tract infections, influenza A, influenza B, human parainfluenza virus 1-4 (HPIV1-4), respiratory syncytial virus (RSV), rubella virus (RV) and Coxsackie virus (CSV), were selected for the development of a multiplex nucleic acid sequence-based amplification (NASBA) assay. Quantifiable measurement utilizing an enzyme-linked oligonucleotide capture (EOC) optical detection method, which was described previously, alleviated the requirement of specialized instrumentation that is commonly used in other molecular techniques. Multiplex NASBA-EOC provided rapid and specific detection of a single virus from a multiplexed group, reducing laboratory testing time and enabling high throughput screening. The uniquely designed primers and probes proved to be highly sensitive and specific, exemplifying the robustness of the multiplex NASBA-EOC technique.
AB - A group of common lower respiratory tract infections, influenza A, influenza B, human parainfluenza virus 1-4 (HPIV1-4), respiratory syncytial virus (RSV), rubella virus (RV) and Coxsackie virus (CSV), were selected for the development of a multiplex nucleic acid sequence-based amplification (NASBA) assay. Quantifiable measurement utilizing an enzyme-linked oligonucleotide capture (EOC) optical detection method, which was described previously, alleviated the requirement of specialized instrumentation that is commonly used in other molecular techniques. Multiplex NASBA-EOC provided rapid and specific detection of a single virus from a multiplexed group, reducing laboratory testing time and enabling high throughput screening. The uniquely designed primers and probes proved to be highly sensitive and specific, exemplifying the robustness of the multiplex NASBA-EOC technique.
KW - Enzyme-linked oligonucleotide capture
KW - Enzymes-linked immunosorbent assay
KW - Influenza
KW - Multiplex
KW - Nucleic acid sequence-based amplification
UR - http://www.scopus.com/inward/record.url?scp=77955096901&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2010.04.027
DO - 10.1016/j.jviromet.2010.04.027
M3 - Journal article
C2 - 20447419
AN - SCOPUS:77955096901
SN - 0166-0934
VL - 168
SP - 251
EP - 254
JO - Journal of Virological Methods
JF - Journal of Virological Methods
IS - 1-2
ER -