TY - JOUR
T1 - Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs
AU - Lu, Guang Hua
AU - Chan, Kelvin
AU - Liang, Yi Zeng
AU - LEUNG, Kelvin S Y
AU - Chan, Chi Leung
AU - JIANG, Zhi Hong
AU - ZHAO, Zhongzhen
N1 - Funding Information:
The authors would like to acknowledge their thanks to Mr. Zhuan Chunhu, Mr. Liu Shujun, Mr. Su Cheng (Gansu Shengtai Traditional Chinese Medicine Development, China), Professor Liu Xiaorui (Agriculture Center of Dinxi District, Gansu, China), Mr. C.K. Tan (International Bio-Tec Research Ltd., Hong Kong), Professor Li Liang (Institute of Mianyang Traditional Chinese Medicine, Sichuan, China), Professor Zhang Hao and Tang Xinyao (West China School of Pharmacy, Sichuan University, Chengdu, China) for collecting some herbal samples. The project was supported by the Faculty Research Grant of Hong Kong Baptist University (FRG/00-01/II-83), the Key Science and Technology Programme of Sichuan Province (02SG011-064, China) and the National Natural Science Foundation of China (20235020, China).
PY - 2005/5/6
Y1 - 2005/5/6
N2 - A high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.
AB - A high-performance liquid chromatographic (HPLC) fingerprint of Chinese Angelica (CA) was developed basing on the consistent chromatograms of 40 CA samples (Angelica sinensis (Oliv.) Diels). The unique properties of this HPLC fingerprints were validated by analyzing 13 related herbs including 4 Japanese Angelicae Root samples (JA, A. acutiloba Kitagawa and A. acutiloba Kitagawa var. sugiyame Hikino), 6 Szechwan Lovage Rhizome samples (SL, Ligusticum chuanxiong Hort.) and 3 Cnidium Rhizome samples (CR, Cnidium officinale Makino). Both correlation coefficients of similarity in chromatograms and relative peak areas of characteristic compounds were calculated for quantitative expression of the HPLC fingerprints. The amount of senkyunolide A in CA was less than 30-fold of that in SL and CR samples, which was used as a chemical marker to distinguish them. JA was easily distinguished from CA, SL and CR based on either chromatographic patterns or the amount of coniferyl ferulate. No obvious difference between SL and CR chromatograms except the relative amount of some compounds, suggesting that SL and CR might have very close relationship in terms of chemotaxonomy. Ferulic acid and Z-ligustilide were unequivocally determined whilst senkyunolide I, senkyunolide H, coniferyl ferulate, senkyunolide A, butylphthalide, E-ligustilide, E-butylidenephthalide, Z-butylidenephthalide and levistolide A were tentatively identified in chromatograms based on their atmospheric pressure chemical ionization (APCI) MS data and the comparison of their UV spectra with those published in literatures.
KW - Angelica spp.
KW - Cnidium officinale
KW - Fingerprint
KW - Ligusticum chuanxiong
KW - Umbelliferae herbs
UR - http://www.scopus.com/inward/record.url?scp=17444416162&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2004.11.080
DO - 10.1016/j.chroma.2004.11.080
M3 - Journal article
C2 - 15909545
AN - SCOPUS:17444416162
SN - 0021-9673
VL - 1073
SP - 383
EP - 392
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 1-2
ER -