Development of an immunochromatographic test strip for the rapid detection of aristolochic acid A in herbal medicinal materials

Xianrui Chen, Ting Huang, Zhibing Huang*, Quanbin Han*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction

Plants containing aristolochic acid and its derivatives are nephrotoxic, mutagenic, and carcinogenic to humans; chronic diet poisoning caused by the aristolochic acid is the cause of endemic (Balkan) nephropathy and related cancers. 

Objective

To develop a colloidal gold immunochromatographic test strip (ICS) based on the competitive format for the rapid detection of aristolochic acid A (AA-A) in herbal medicinal materials. 

Materials and methods

For the ICS based on gold nanoparticles (AuNPs), the antigen [AA-A–bovine serum albumin (BSA)], and goat anti-mouse IgG were drawn on the nitrocellulose membrane as the test line (T line) and the control line (C line), respectively. Monoclonal antibody (MAb)–AuNP conjugates were sprayed onto the conjugate pad. The sensitivity of the ICS was 6 ng/mL, and the test was completed in 10 min. The analysis of AA-A in traditional Chinese medicine samples showed that the ICS results were in good agreement with those obtained by high-performance liquid chromatography methods. 

Conclusion

These results demonstrated that the ICS test could be used as a reliable, rapid, cost-effective, and convenient qualitative tool for on-site screening techniques to detect AA-A in herbal medicinal materials without any special instrumentation.

Original languageEnglish
Pages (from-to)441-451
Number of pages11
JournalPhytochemical Analysis
Volume33
Issue number3
Early online date21 Nov 2021
DOIs
Publication statusPublished - Apr 2022

Scopus Subject Areas

  • Analytical Chemistry
  • Food Science
  • Biochemistry
  • Molecular Medicine
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

User-Defined Keywords

  • aristolochic acid
  • gold nanoparticle
  • immunochromatographic test strip
  • monoclonal antibody
  • traditional Chinese medicine

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