@article{6d7cc55b99534edfa56a664b029c72b0,
title = "Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation",
abstract = "We report a label-free, structure-independent luminescent-sensing platform for metal ions, proteins, and small molecules utilizing an Ir(III) complex, terminal deoxynucleotidyl transferase (TdT), and a structure-folding aptamer. A novel G-quadruplex-selective Ir(III) complex was identified to detect the nascent G-quadruplex motifs with an enhanced luminescence response. Unlike most label-free DNA-based assays reported in the literature, this sensing platform does not require a specific secondary structure of aptamer, thus greatly simplifying DNA design. The detection platform was demonstrated by the detection of K+ ions, thrombin, and cocaine as representative examples of metal ions, proteins, and small molecules.",
keywords = "G-quadruplex, iridium(III) complex, label-free, TdT, universal detection",
author = "Leung, {Ka Ho} and Bingyong He and Chao Yang and Leung, {Chung Hang} and Wang, {Hui Min David} and Ma, {Edmond Dik Lung}",
note = "This work is supported by Hong Kong Baptist University(FRG2/14-15/004), the Health and Medical Research Fund(HMRF/13121482 and HMRF/14130522), the Research Grants Council (HKBU/201811, HKBU/204612, and HKBU/201913), the French National Research Agency/Research Grants Council Joint Research Scheme (A-HKBU201/12), State Key Laboratory of Environmental and Biological Analysis Research Grant (SKLP-14-15-P001), National Natural Science Foundation of China (21575121),Guangdong Province Natural Science Foundation(2015A030313816), Hong Kong Baptist University Century Club Sponsorship Scheme 2015, Interdisciplinary Research Matching Scheme (RC-IRMS/14-15/06), the State Key Laboratory of Synthetic Chemistry, the Science and Technology Development Fund, Macao SAR (103/2012/A3, 098/2014/A2, and 007/2014/AMJ), and the University of Macau(MYRG091(Y3-L2)-ICMS12-LCH, MYRG2015-00137-ICMS-QRCM, and MRG023/LCH/2013/ICMS).",
year = "2015",
month = nov,
day = "4",
doi = "10.1021/acsami.5b08314",
language = "English",
volume = "7",
pages = "24046--24052",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "43",
}