Development of an Aptamer-Based Sensing Platform for Metal Ions, Proteins, and Small Molecules through Terminal Deoxynucleotidyl Transferase Induced G-Quadruplex Formation

Ka Ho Leung*, Bingyong He, Chao Yang, Chung Hang Leung, Hui Min David Wang, Edmond Dik Lung MA

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

We report a label-free, structure-independent luminescent-sensing platform for metal ions, proteins, and small molecules utilizing an Ir(III) complex, terminal deoxynucleotidyl transferase (TdT), and a structure-folding aptamer. A novel G-quadruplex-selective Ir(III) complex was identified to detect the nascent G-quadruplex motifs with an enhanced luminescence response. Unlike most label-free DNA-based assays reported in the literature, this sensing platform does not require a specific secondary structure of aptamer, thus greatly simplifying DNA design. The detection platform was demonstrated by the detection of K+ ions, thrombin, and cocaine as representative examples of metal ions, proteins, and small molecules.

Original languageEnglish
Pages (from-to)24046-24052
Number of pages7
JournalACS applied materials & interfaces
Volume7
Issue number43
DOIs
Publication statusPublished - 4 Nov 2015

Scopus Subject Areas

  • Materials Science(all)

User-Defined Keywords

  • G-quadruplex
  • iridium(III) complex
  • label-free
  • TdT
  • universal detection

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